Lysobacter humi sp. nov., isolated from soil.

Abstract

A yellow-pigmented and strictly aerobic bacterial strain, designated FJY8T, was isolated from the soil of Goyang, South Korea. The cells of FJY8T were Gram-reaction-negative, non-motile rods. Colonies were circular, convex and transparent. Strain FJY8T grew optimally at 30 °C, with 0 % (w/v) NaCl and at pH 8. Phylogenetic analysis of the 16S rRNA gene sequence of FJY8T revealed a clear affiliation of this bacterium to the family Lysobacteraceae, and it was related to members of the genus Lysobacter, with Lysobacter xinjiangensis KCTC 22558T being its closest relative (98.7 % sequence similarity). The DNA G+C content was 68.0±0.4 mol%. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were identified as the major polar lipids, and an unidentified phospholipid and two unidentified aminophospholipids were also detected as the minor polar lipids. The major fatty acids were iso-C16 : 0, summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C15 : 0. Only ubiquinone-8 (Q-8) was detected as the isoprenoid quinone. DNA-DNA hybridization values of strain FJY8T with Lysobacter xinjiangensisRCML-52T and Lysobacter mobilis9NM-14T were 55.8±2.0 and 45.2±4.8 %, respectively. On the basis of DNA-DNA hybridization, phylogenetic distinctiveness, and some physiological and biochemical tests, strain FJY8T (=KCTC 42810T=JCM 31019T) represents a novel species of the genus Lysobacter, for which the name Lysobacter humi sp. nov. is proposed.