Abstract
Two programmes for analysis of enzyme kinetic data are described. The programmes have evolved from use in undergraduate classes and accordingly the emphasis is on simplicity in design and use, rather than on computational sophistication. Data fitting, to a selection of predetermined models, proceeds via a weighted linear regression on to the reciprocals of the substrate concentrations and catalytic rates and is rapid and robust. The programmes yield best-fit valus for Km, Vm and, when appropriate, Ki together with measures of the goodness of fit. Experimental data and lines of best fit may be presented graphically in up to seven diifferent plotting methods. Entered data and the graphical and numerical results of model fitting are displayed on the same screen so that relationships between all three may be readily appreciated. Variants of the programmes may be employed in the Windows 3× or 9× platforms or in DOS.
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