<i>Microccus gluamicus</i> の細胞学的研究

Abstract

It was reported in a previous paper that the elongated, enlarged and even branched form of Micrococcus glutamicus was observed in the synthetic medium containing Na-citrate or Na-malate. As the result of the investigations on substances having the effect like that of Na-citrate or Na-malate, following informations were obtained obviously.1. Among various salts of citrate, ammonium and potassium salts showed the similar effect to that of Na-salt on the morphological change.2. Na-oxalate also has a strong elongating and branching effect.3. Generally, amino acids promote the cell growth of M. glutamicus. The large quantities of L-alanine and L-cystein, however, inhibit its growth. The elongated and enlarged cells were formed by the addition of 50mg./ml. of L-cystein.4. High multicellular and elongated bodies were formed in a medium containing the low level of penicillin. Such multicellular bodies, however, were never formed in the penicillinmedia if the biotin content was increased to 100γ/l. From this fact, it seems that penicillin strongly inhibits the separation of the daughter cells rather than the cytoplasmic division.5. The multicellular bodies never formed in a medium containing streptomycin. But a vacuole-like structure in each cell was formed in the concentration of 0.4-0.8γ/ml. of streptomycin.6. Remarkable multicellular bodies were formed in the concentration of 0.2-0.8γ/ml. of mitomycin-C. In these cases, the length of cell is 10μ or over occassionally. One or both ends of these cells were swollen and become so-called club-shape.