<i>Mazaediothecium keibulense</i> (Pyrenulaceae), A new Lichen species from Manipur, India

Keibul Lamjao National Park (KLNP), Loktak lake Manipur, north-east India which is one of the 25 Ramsar sites of international importance and the biggest fresh water Lake in India. Macrophytes of the floating Phumdi mat environment of KLNP play an important role in the aquatic environment. An investigation was planned during the period of about three years from March, 2010 to December 2012, to study the diversity pattern of habitats of macrophytes available in six study sites of KLNP viz., 1.Kumbi, 2.Khordak, 3.Keibul, 4.Toya, 5. Nongmaikhong and 6. Sargam. All total 85 dominant plant species were recorded. Maximum plant species (49) was observed in Site-1 Kumbi (Altitude-780m) and minimum (27) in Site-3 Keibul (Altitude-772). The variation of plant species may be because of the slight variation of altitude. As the six study sites are distributed as floating aquatic environment in KLNP Loktak Lake, there is favourable place of the plants in higher altitude and altitude represents a complex gradient along which many environmental variables change concomitantly. In all the six study sites, common distribution of ten dominant plant macrophytes viz., Ageratum conizoides, Hedychium coronarium, Leersia hexandra, Oenanthe javanica, Phragmites karka, Polygonum sagittatum, Saccharum munja, Thelypteris interrupta and Zizania latifolia was noticed. Individual dominant plant was recorded in other sites also viz., Azola piñata in site-6; Xanthium atrumarium, Polygonum orientale, Dichrocephala latifolia and Cymbopogon citratus in site-2; Arundo plinii, Cuscuta reflexa, Gnaphalium luteo-album, Hydrilla verticillata, Marsilia minuta, Saccolepis interrupta, Selvenia cuculata and Utricularia spp. in site-1. Variation of IVI value of dominant plant species was observed in this study. Even though 85 dominant plants were selected for all the 6 sites, however, maximum plant richness expressed in IVI was recorded in case of Zizania latifolia (Site-5, IVI-87.5) and minimum IVI in case of Xanthium strumarium (Site-2, IVI-1.13). The diversity pattern and habitats of macrophytes in KLNP Phumdi environment might be due to water availability along the altitudinal gradient and other environmental factors suited in the study sites and expected to be an important factor affecting the survival and fecundity of plant population. We need to conserve the natural habitat of KLNP thereby maintaining the luxuriant growth of the seasonal and perennial, macrophytes, so as to maintain the natural flora and fauna of the park. These plants are the food of man and animal therefore, it needs a proper care and attention to protect them from over exploitation. Considering the diversity pattern of habitats of Macrophytes in KLNP, a floating mat Phumdi environment with high floral diversity and unique vegetation assemblage, it has been suggested that this track and adjoining sites should be declared as ecologically sensitive area not only the World Heritage Site.

Assessing seasonal variation of diffusive nitrous oxide emission from freshwater wetland in Keibul Lamjao National Park, Manipur Northeast India

The hog deer (Axis porcinus) is threatened by habitat alteration, fragmentation, and poaching, which have led to a drastic decline of its wild population. Two subspecies of A. porcinus have been described from its distribution range. A. p. porcinus is reported to occur from Pakistan along the Himalayan foothills through Nepal, India and Myanmar, and A. p. annamiticus is found in Thailand, Indo-China, Laos, Cambodia, and Vietnam. However, the current distribution range of A. p. annamiticus is still unclear. We used the partial control region (CR) of mitochondrial DNA (mtDNA) and seven microsatellite loci to investigate the intra-species structure, differentiation, and demographic history of hog deer populations from three landscapes, the Terai Arc, Northeast, and Indo-Burma (Keibul Lamjao National Park (KLNP), Manipur, India) landscapes. We also carried out divergence time estimation using the complete mitogenome. The level of variation was ~4%, and the time of divergence of the KLNP population and the other Indian populations was about 0.22 Mya, i.e., during the last glaciation periods of the Late Pleistocene/Early Holocene. The KLNP haplotypes of the control region were shared with the Southeast Asian subspecies, A. p. annamiticus. The results of the investigations of the microsatellite loci supported the mtDNA results unambiguously. Two genetically distinct lineages are found in India: one is found from the Terai Arc to Assam (A. p. porcinus) and the other in Manipur (A. p. annamiticus). The genetic diversity in KLNP was low and exhibited a higher degree of genetic differentiation compared with major Indian populations. The Bayesian skyline plots indicated that after a long phase of historic demographic stability, the populations of both the lineages of hog deer suffered pronounced declines during the period from ~800 years BP to 5000 years BP. In summary, our finding provided evidence that the KLNP population is probably a prime, isolated and sustaining stock of A. p. annamiticus and should be managed as evolutionarily significant units (ESUs).

Aquatic insect diversity of a protected area, Keibul Lamjao National Park in Manipur, North East India

The Sangai deer (Rucervus eldii eldii) is an endangered species which is found only in KeibulLamjao National Park, Manipur (India) with an area of 40 km2. Selection of observation point and direct observation method were utilized to record certain behavioral pattern shown by the Sangai deer. Determination of food resources were carried out to identified feed species during the observation hour. The finding of the present study shows that the behavioral activities were notice more in the morning (dawn) than evening (dusk). The most favorable food by Sangai deer were Zizania latifolia (Kambong), Pragmitis karka (Tou), Saccharum munja (Khoimom), Carex cruciata (Humdang), Learsia hexandra (Hup), Alpinanigra (Pullei) and Hydechiumcoronarium (Loklei). Amongst the favorable food species, the most preferable food by the Sangai deer is Zizania latifolia while some of the individuals were seen consuming ashes (Tanggoi) during the observation. Intrusion of domestic animals within the park area may lead to severe calamities which then lead to the spread of many harmful diseases to Sangai deer and its inhabitants. Shyness of animal and sensitive behaviors of Sangai deer was observed. Questionnaire surveys were carried out among 40 respondents. The result suggested that the status of feeding must be taken care so that there should not be any alteration or changes in their behavior due to intrusion, local vegetables collector and disturbances.

Seasonal variations in the water quality of a tropical wetland dominated by floating meadows and its implication for conservation of Ramsar wetlands

An in-depth understanding of carbon dynamics and ecosystem productivity is essential for conservation and management of different ecosystems. Ecosystem dynamics and carbon budget are assessed by estimating net ecosystem production (NEP) across different global ecosystems. An ecological productivity assessment of forest and floating meadow ecosystems in Keibul Lamjao National Park (KLNP), Manipur, North East India, was conducted using the multi-criteria decision-making process namely, gray relational analysis (GRA). The analysis was performed on 24 selected criterions classified either as "higher-the-better" or "lower-the-better" based on their degree of influence on the carbon budget. Floating meadows exhibited a higher production of aboveground and belowground biomass and a higher total mortality and decay. Furthermore, the study found that floating meadows exhibited a higher soil organic carbon (SOC) and net soil organic matter (SOM) than the forest ecosystem. The forest ecosystem showed higher total respiration (RT), heterotrophic respiration (RH), and autotrophic respiration (RA) than floating meadows. Floating meadows exhibited a higher net primary productivity (NPP) of 616.49 ± 33.87 gCm-2year-1 than the forest ecosystem, which has a NPP of 566.64 ± 65.26 gCm-2year-1. Similarly, floating meadows have higher NEP (495.25 ± 36.46 gCm-2year-1) than forest ecosystems (418.39 ± 65.76 gCm-2year-1). These characteristics have a significant influence on the carbon budget in floating meadows as compared to forest ecosystems, as shown by larger values of gray relational coefficient (GRC) in GRA. The floating meadows ecosystem (0.82) obtained 54.72% gain in gray relational grades (GRG) value with the forest ecosystem (0.53). This study might help in improving KLNP and other adjutant areas for conservation and management policies from the vital information given on the importance of wetlands in carbon dynamics and ecosystem productivity.

Loktak Lake is the largest fresh water Lake in the North-eastern India. Keibul Lamjao National Park (KLNP) is a floating-mat (Phumdi) park inside the Lake. Study deals with the inter-related approaches: micobiological study, free-listing, participant observation and preference ranking of the six study sites of KLNP. Generally, plant collection per day was about 600 kg of Hedychium coronarium J. Koenig which were extracted from the park and found to be the most preferable species; while Persicaria sagittata (L.) H. Gross, as the least among the selected plants. Microbiological analysis of water for the six study sites recorded high pollution as evidenced by high values of Standard Plate Count (SPC) for bacteria ranging from 73,500-96,500 and microbial analysis of MPN (Most Probable Number) of coliform bacteria varies between100 and 320/100 ml and faecal coliform 95 and 200/100 ml. Free CO2 concentrations varied at the surface from 2-60 mg/l and 6-70 mg/l at the bottom of the park. In the KLNP environment the higher CO2 and lower concentrations of dissolved oxygen (DO) might be due to the floating mat (Phumdi) nature, in which light and exchange of gases does not occur as it does in the natural water bodies.

As a part of the Asian Waterbird Census (AWC), surveys on ducks and geese were made at five (5) major waterbird congregation sites of Keibul Lamjao National Park (KLNP) during 2020-2024. A total of 18 species of ducks and two species of geese belonging to the family of Anatidae were recorded during the period. The duck species includes 14 migratory and 4 resident species and the two migratory geese species. Lesser Whistling Duck Dendrocygna javanica was the most abundant and dominant species in terms of population followed by Gadwall Anas strepera and Red - crested Pochard Netta rufina and minimum value was observed in Greylag Goose Anser anser. The Critically Endangered Baer’s Pochard Aythya fuligula was recorded in the year 2023 at Pabot Chingmang site (2 individuals). Another two globally Near threatened (NT) Ferruginous Pochard Aythya nyroca and Falcated Duck Anas falcata have been recorded here.

Keibul Lamjao National Park (KLNP), a floating park in Loktak Lake, Manipur (India) was studied from Winter (WIN) to Post Monsoon (POM) for its zooplankton composition and some selected water parameters. The resultant data were subjected to multivariate techniques–Principal Component Analysis (PCA) and Canonical Correspondence Analysis (CCA). Analyses of water parameters with PCA revealed that the first PC axis (PC1) accounts for maximum variance in the seasonal data, explaining a variability of 91%. The PCA revealed that the seasonal variability in water parameters was due to the wet and dry cycle of seasons and the stations were distinguished on the basis of transparency and turbidity. Zooplankton abundance was dominated by copepods followed by cladocerans. Temporally, abundance of copepods reached a maximum during Post-monsoon (POM) (3 880 ind./L). Spatially, S6 was found to be most abundant of the other stations in zooplankton. Copepodites and nauplii larvae were the major components of zooplankton. The Rotifera were the least abundant among the three zooplankton groups. Brachionus formed the major component of Rotifera zooplankton at all the stations during the study period. In the Cladocera, Macrothrix was present during all the four seasons, while Pleuroxus, Oxyurella, Kurzia and, Diaphanosoma were rare. The CCA shows that maximal temporal variability in zooplankton abundance was explained by temperature and rainfall. ANOVA revealed no significant diff erence in mean zooplankton abundance among the seasons, but there was a statistically significant diff erence among the sites.

As a part of the monitoring practice of the lone natural habitat of the endemic and endangered Rucervus eldii eldii McClelland or Sangai, the paper reports sediment and water characteristics of Keibul Lamjao National Park-the only floating national park of the world in Manipur, Indo Burma hotspot. The water analysis showed low pH (5.69 ± 0.16), high electrical conductivity (342 ± 13.01 μS m-1), high turbidity (33.2 ± 9.407 NTU), and high phosphate concentrations (0.92 ± 0.11mg L-1) during the study period. The water quality index values calculated indicate that the park water during post-monsoon season is unfit for drinking. Thus, the degrading water quality poses a serious risk to the health of the deer and other species in the park. At present, Sangai in its natural habitat is threatened by pollution, encroachment, phoomdi thickness reduction, and inbreeding depression. To address the problem of inbreeding, Pumlen pat is considered as a second suitable natural habitat for the deer reintroduction program. During the study, water characteristics of the wetland showed similar characteristics as KLNP, i.e., low pH (5.86 ± 0.30), high electrical conductivity (377.6 ± 5.55 μS m-1), high turbidity (32.36 ± 4.91 NTU), and high phosphate concentrations (0.79 ± 0.14mg L-1). Likewise, sediments showed high TP accumulation that ranged from 197.03 ± 0.75 to 332.88 ± 0.99mgkg-1 for KLNP and 245.18 ± 0.85 to 351.48 ± 0.71mgkg-1 for Pumlen pat, respectively. Both, the lone natural habitat and the proposed habitat showed deteriorating water quality. As such, continuous monitoring of KLNP and Pumlen pat water and sediment quality should be a priority during the management practices to protect the endangered deer and health of the habitats for long-term conservation.

Odonata larvae were collected from Keibul Lamjao National Park, Manipur, northeastern India during 2009-2011. The study recorded 15 species of Odonata larvae belonging to the anisopteran families Aeshnidae, Libellulidae and zygopteran families Calopterigidae, Lestidae and Coenagrionidae. The study revealed that there is a need for a detailed study of Odonata fauna of the national park as thorough knowledge of the important species of a conservation area is very essential for proper management.

A wetlandscape is a landscape characterized by the presence of numerous interconnected wetlands (Bertassello et al. 2018). Loktak located in the Manipur River basin in India is a unique wetlandscape that includes numerous floodplain wetlands and associated channels. It hosts the Loktak wetland, the largest freshwater body in the northeastern India, also designated as Ramsar site and the Keibul Lamjao National Park, the only floating national park in the world. Like any other wetlands in the world, Loktak wetlandscape is also under great threat due to the changes in the hydrometeorological conditions associated with climate change as well as the human interventions in the wetlandscape and its catchment. Loktak hydro-electric project, the major anthropogenic intervention in the wetlandscape has a huge impact on the hydrology and ecology of Loktak wetland complex and to Pumlen wetland complex to a less significant level (Trisal and Manihar 2002). This work investigates the multi- and cross-scale degradation of wetlands in the Loktak wetlandscape in a nested-framework by studying hydrogeomorphic dynamics at catchment scale, wetlandscape scale, and wetland complex scale. The catchment scale encompasses surrounding uplands and hillslopes. At the wetlandscape scale, which includes interconnected wetlands and associated channels, individual wetland boundaries are demarcated using historical Corona image and recent Sentinel-2 image. By comparing historic and recent boundaries, we found that six natural wetlands of area less than 1.5km2 have completely converted into other land use types and three small wetlands with area less than 1km2 get merged with bigger ones due to barrage-induced prolonged inundation. The wetland complex scale is a cluster of hydrologically interconnected wetlands of same or different types. Loktak wetland complex has not shown any prominent change in its extent, whereas Ikop and Pumlen wetland complexes have shrunken remarkably over time. The degradation of wetlandscape is evident from other factors such as proliferation and thinning of phumdis (floating biomass) and destruction of vegetation in the catchment. The cross-scale investigation suggests the influence of both natural and anthropogenic controls on the degradation of Loktak wetlandscape. The findings of this study and the protocols developed here will help to better understand the stressors of Loktak wetlandscape and elsewhere and could be instrumental in developing a conservation and management plan. Multi-scale management of wetlandscape include the catchment-scale measures such as afforestation, protection of hills and reducing the frequency of shifting (jhum) cultivation in hilly areas, wetlandscape scale measures such as control of waste dumping, control of channel modification for builtup and then wetland scale measures such as removal of invasive species from open water, and control of athaphum farming (method of farming using phumdis). Considering that a huge proportion of population in the valley depend on these wetlands for their livelihood, accounting their needs and making them a part of any effort for the management of this ecosystem has to be one of the primary goal.

Manipur is a biodiversity rich state located in the northeastern part of India that borders Myanmar. Situated within the western portion of the Indo-Burma biodiversity hotspot, the state has a large number of endemic and endangered species. The state is also prone to habitat destruction due to rapid clearing of forest for shifting cultivation, which is a common practice in the hill districts for agriculture and collection of firewood and timber. In the valley districts, the entire forest areas were converted to agricultural fields leaving only a few remaining green spaces, such as the sacred groves locally known as Umang Lais, small hillocks, and Keibul Lamjao National Park.

Ecological factors that control the species distribution patterns at various spatiotemporal scales will get affected by climate change. To combat the situation, in the past few decades geographical information system (GIS) and remote sensing have been widely used by the researchers in the field of wildlife and habitat suitability modeling. The main objective of this study is to map and predict the current and future habitat suitability potential of Rucervus eldii eldii in Keibul Lamjao National Park (KLNP) using MaxEnt. Presence location data of the species, topographic factors, and bio-climatic variables were used as input in the MaxEnt software to map current habitat suitability potential. To map the habitat suitability potential for future, two representative concentration pathway (RCP) scenarios RCP 2.6 and RCP 8.5 for the years 2050 and 2070 were used. The model returned an average AUC value of 0.944 which indicates the model to be sensitive and descriptive. Isothermality and precipitation in the wettest quarter were found to be two most significant variables. The suitable range of precipitation in the wettest quarter for Rucervus eldii eldii varies from 1365 to 1410mm with an optimal value of 1405mm and isothermality from 46.43 to 46.6% with an optimal value of 46.5%. Current habitat suitability results of the model show 0.45 km2 of the area under no potential, 29.25 km2 of the area under least potential, 8.29 km2 of the area under moderate potential, 9.21 km2 of the area under good potential, and 8.82 km2 of the area under high potential. Both RCPs for the years 2050 and 2070 show the decreasing trend in the area under high suitability potential and increasing trend under no suitability potential. The results of this study can provide aid in the management and protection of Rucervus eldii eldii.