Abstract
<p class="Abstract"><em>Canthium </em>parviflorum is used in the traditional therapy to treat snakebite victims. In the present study, partially purified methanol root extract of <em>C. </em>parviflorum was used for the neutralization of Eachis carinatus and <em>Naja naja</em> venom. The extract inhibited <em>in vitro</em> phosphomonoesterase, phosphodiesterase, acetylcholinesterase, hyaluronidase, protease, phospholipase A<sub>2 </sub>and 5’nucleotidase activities of both venoms. One of the promising band exhibited neutralization of all the<em> in vitro</em> enzyme activities and was further subjected to GC-MS analysis which revealed the presence of eight active phytocompounds. These phytochemicals might be responsible for <em>in vitro</em> enzyme neutralization. Methanol extract possesses potent active phytochemicals against the site specific toxins and hydrolytic enzymes analyzed.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract"><a href="https://youtube.com/v/oSLdFcfaJxU">Phospholipase A2 assay</a>: 1 min 36 sec</p>
Highlights
World Health Organization highlights snakebite as a “Neglected disease”
The present study focuses on the antivenom potential of thin layer chromatography (TLC) separated methanol extract of plant against E. carinatus and N. naja venom
Di-sodium-pnitrophenol phosphate (DNPP), L-leucine, diansidine hydrochloride, horseradish peroxidase, 5,5-dithiobis-(2nitrobenzoic acid) (DTNB), acetylthiocholine iodide, hyaluronic acid, cetyltrimethylammonium bromide and lecithin were purchased from Himedia Laboratories and casein from Sigma-Aldrich laboratories, USA
Summary
World Health Organization highlights snakebite as a “Neglected disease”. It is a medical emergency in the South East Asian Region including India (WHO, 2007; Banerjee, 1978). There were 52 poisonous snakes reported in India of which four species (Naja naja, Bungarus caeruleus, Daboia russelii and Echis carinatus) are responsible for mortality and maximum deaths (Bawaskar 2004). The most effective and accepted therapy for snakebite patient is the immediate administration of polyvalent anti-venom after the envenomation which is associated with several drawbacks. Antivenom binds and neutralizes the venom, further preventing the adverse effects due to venom. It cannot reverse the damages already caused by the venom (Gomes et al, 2010)
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