Abstract
Purpose: To investigate the effects of very low-frequency whole body vibration (WBV) stimulation using PC12 m3 cells. Methods: Mutant, drug-hypersensitive, PC12 m3 cells were obtained by means of continuous culture of neural PC12 cells and then stimulated by low-frequency vibration at frequencies of 7–13 Hz for 5–20 min with simultaneous exposure to nerve growth factor (NGF). Results: The frequency of neurite outgrowth by PC12 m3 cells induced by 7-Hz vibratory stimulation was approximately 3-fold greater than that induced by NGF alone. Moreover, activated cyclic-AMP responsive element (CRE)-binding protein (CREB) expression was induced in PC12 m3 cells stimulated by 7-Hz vibration. Conclusion: Low-frequency vibratory stimulation induces neurite outgrowth via a p38 mitogen-activated protein kinase/CREB signaling pathway in PC12 m3 cells. Together, these results suggest that WBV is an efficient method to stimulate neurite growth.
Highlights
Recent studies have reported that whole body vibration (WBV) was effective for improving bone strength, muscle strength in the lower extremities, and functional mobility [1,2,3]
We examined whether the ability of a lowfrequency vibratory stimulus to induce neurite outgrowth of PC12 m3 cells is a reflection of its effect on p38 mitogen-activated protein kinase (MAPK) activity [5]
Because cyclic AMP-responsive element binding protein (CREB) is a transcription factor that plays an important role in promoting cellular proliferation and adaptive responses, a mechanism has been defined by which Vascular endothelial cell growth factor (VEGF)/kinase insert domain receptor (KDR) may allow endothelial cells to respond to changes in their environment through alterations in their gene expression [24]. These findings indicate that by mediating cellular responses to VEGF, CREB, through its activation of p38 MAPK/ mitogen-and stress-activated protein kinase-1 (MSK-1) signaling pathways, is likely to play an important role in endothelial cell function and blood development
Summary
Recent studies have reported that whole body vibration (WBV) was effective for improving bone strength, muscle strength in the lower extremities, and functional mobility [1,2,3]. The effects of very low frequency vibrations are uncertain. The purpose of our study was to determine the effects of very low-frequency vibratory stimulation to induce neurite outgrowth by PC12m3 cells. The effects of each treatment were examined using PC12 m3 cells before the initiation of the clinical study [5,6]. The “steam foot spa” treatment resulted in improved cognitive function and a temporary decrease in high blood pressure in geriatric inpatients [8]. The effect of this treatment was confirmed in PC12 m3 cells and further observed in ongoing clinical investigations
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
