Abstract
Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.
Highlights
Mesenchymal stromal cells have potential in regenerative medicine and modulating the immune system
Cell cryopreservation has been achieved with polyampholytes using slow-cooling methods, which are desirable due to low solvent concentrations, sample stability, and straightforward processes
We recently reported a polyampholyte cryoprotectant that was a potent additive for the cryopreservation of cell monolayers using slowcooling, which is an extremely challenging model.[21]
Summary
Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system.
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