Abstract
A new needle nematode, Longidorus carniolensis sp. n., recovered from the soil around the roots of grapevine Vitis vinifera L. from Slovenia, is described and illustrated. Longidorus carniolensisis an amphimictic species, characterised by females with a moderately long (L=5.6–8.2 mm) and plump (a=51–72.4, ave. 66.3) body, assuming a spiral to C-shape when heat relaxed. Head region continuous, anteriorly almost flat, lip region 23–25 µm wide; guiding ring situated posteriorly (42–47 μm, 43–50 μm in males), odontostyle long (ave. 146.6 (136–157) μm); pharyngeal glands with normal location, their nuclei of approximately equal size; tail bluntly conoidal to almost hemispherical. Males abundant, spicules slender and long (122–145 μm), ventromedian supplements 13–17, irregularly spaced, preceded by an adanal pair. Four juvenile stages present, the first stage juvenile with bluntly conoidal tail. Codes for identifying the new species when using the key by Chen et al. (1997) are: A 56, B 4, C 4, D 1, E 4, F 35, G 1, H 1, I 2. The new species is morphologically the most similar to Longidorus poessneckensis Altherr, 1974, Longidorus macrosoma Hooper, 1961, Longidorus caespiticola Hooper, 1961, Longidorus helveticus Lamberti et al., 2001, Longidorus macroteromucronatus Altherr, 1974, Longidorus pius Barsi & Lamberti, 2001, Longidorus raskii Lamberti & Agostinelli, 1993, Longidorus kheirii Pedram et al. 2008, Longidorus silvae Roca, 1993, Longidorus iuglandis Roca et al., 1985, Longidorus vinearum Bravo & Roca, 1995 and Longidorus major Roca & d’Erico, 1987, but differs from these species either by the body and odontostyle length, position of guide ring, head region and tail shape or the shape of the first stage juvenile tail. Sequence data from the D2-D3 region of the 28S rDNA distinguishes this new species from other speciesof the genus Longidorus with known sequences. Relationships of Longidorus carniolensis sp. n. with other Longidorus species based on analysis of this DNA fragment and morphology are discussed.
Highlights
The nematodes of the genus Longidorus Micoletzky, 1922 cause damage to many economically important crops by direct feeding on their roots
Six Longidorus species have been reported from Slovenia (Širca and Urek 2009): L. elongatus Micoletzky, 1922, L. caespiticola Hooper, 1961, L. juvenilis Dalmasso, 1969, L. helveticus Lamberti, Kunz, Grunder, Molinari, De Luca, Agostinelli & Radicci, 2001, L. leptocephalus Hooper, 1961, L. moesicus Lamberti, Choleva & Agostinelli, 1983
There are some characteristic morphological features observed in L. carniolensis sp. n. such as the presence of vestigium, hemizonid and hemizonion, and the abberant ventral pore
Summary
The nematodes of the genus Longidorus Micoletzky, 1922 cause damage to many economically important crops by direct feeding on their roots. N. The description of the new species is based both on morphological and molecular data, in particular the sequence of D2D3 expansion regions of the large subunit rDNA nuclear gene which proved to be useful in molecular phylogenetic analyses of Longidoridae (Rubtsova et al 2001, Ye et al 2004, He et al 2005). The description of the new species is based both on morphological and molecular data, in particular the sequence of D2D3 expansion regions of the large subunit rDNA nuclear gene which proved to be useful in molecular phylogenetic analyses of Longidoridae (Rubtsova et al 2001, Ye et al 2004, He et al 2005) Sequences of these domains allow species differentiation (Širca and Urek 2009)
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