Abstract
Objective To explore the mechanism of cell apoptosis of immortalized human keratinocytes (HaCaT cells) and protein expression related to this process after long term exposure to sodium arsenite (NaAsO2, 1.0 μmol/L). Methods Malignant transformation model was set up through long-term exposure of HaCaT cells to 1.0 μmol/L NaAsO2. Cell passage for 0, 1, 7, 14, 21, 28 and 35 generations in the process of malignant transformation were collected for measurement of cell apoptosis rate by flow cytometry, and apoptosis related proteins by Western blotting, including activation of cysteine protease 3, 8 (cleaved-caspase-3, 8), C/EBP homologous protein (CHOP), B-cell leukemia/lymphoma 2 (Bcl-2), and Bcl-2 associated X protein (Bax). Results Along with the arsenite treatment, the apoptosis levels were significantly decreased (F= 26.770, all P 0.05). Conclusion In the process of malignant transformation, the apoptosis levels of HaCaT cells are inhibited after long term sodium arsenite exposure through mitochondria and endoplasmic reticulum (ER) stress signaling pathways. Key words: Arsenites; HaCaT cell; Apoptosis
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