Abstract
The aim of the present study was to verify the long-term cytotoxic effects of the MTA Fillapex and to compare them with AH Plus. Dissolution rate and pH were also evaluated. Human osteoblast cells were incubated with elutes of fresh specimens from AH Plus and MTA Fillapex, and with elutes of the same specimens for 4 successive weeks. Elute's pH was evaluated at each time point. A multiparametric cell viability assay was performed. For dissolution rate, ISO methodology was used. The results were analyzed by one-way analysis of variance, complemented with the Tukey post-test (p<0.05). No significant difference was found among the materials when fresh mixed (p>0.05). After 1 week, AH Plus became non-cytotoxic on all three evaluated parameters. Conversely, MTA Fillapex remained severely and mildly cytotoxic over the entire experimental period (p<0.05). The dissolution rate of AH Plus was significantly lower than MTA Fillapex at all time points (p>0.05). The pH of AH Plus was significantly lower than MTA Fillapex at the second and third week (p<0.05). In the other tested time points no statistical difference was observed. In conclusion, MTA Fillapex remained cytotoxic after 4 weeks and its cytotoxicity may be related to the high dissolution rate of this material.
Highlights
In the last decade, mineral trioxide aggregate (MTA), a calcium silicate–based material, became popular in endodontics as a root-end filling and pulp-capping material for the repair of root canal perforations and for apexification [1,2]
MTA Fillapex (Angelus, Londrina, PR, Brazil), a sealer based on calcium silicate, was introduced recently
The results of the human osteoblasts (hOB) viability, as measured by strategies on these materials that differ from most previous the multiparametric cytotoxicity assay over the entire works
Summary
Mineral trioxide aggregate (MTA), a calcium silicate–based material, became popular in endodontics as a root-end filling and pulp-capping material for the repair of root canal perforations and for apexification [1,2]. Despite its favorable characteristics, MTA does not exhibit the physical properties required for an endodontic sealer, because of its working time, setting time and difficult handling [3]. A recent study showed suitable radiopacity, pH and flow of MTA Fillapex [4]. The biocompatibility of endodontic sealers is an important factor in choosing the best material, because endodontic sealers are often placed in intimate contact with the periapical tissues for an extended period. Due to these controversial results, more studies about MTA Fillapex cytotoxic behavior should be performed before its clinical indication
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