Abstract
Long PCR was used to amplify a 5-kb fragment of the bacterial ribosomal operon (16S–intergenic spacer region (ISR)–23S) from several Ralstonia eutropha strains (16S rDNA sequence similarity: 97–99%). Due to the large product size, amplicons from the different strains could be distinguished using restriction enzyme fragment length polymorphisms (RFLP) and repetitive PCR analysis (Rep-PCR) with the primer 1492r. These methods may prove useful in differentiating other bacterial strains with highly similar 16S rDNA sequences.
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