Abstract
Glioma is the most prevalent solid tumor in the central nervous system (CNS). Recently, it has been indicated that long non-coding RNAs (lncRNAs) substantially adjust the development of a variety of human cancers. In the present study, it was found and verified via microarray analysis that lncRNA PSMA3-AS1 exhibited a high expression in glioma tissues and cell lines. Then CCK-8, 5-Ethynyl-2′-deoxyuridine (EdU) staining, plate clone assay, Transwell assay, Western blotting and nude mouse model were adopted to verify PSMA3-AS1’s effects on glioma. Knockdown of PSMA3-AS1 inhibited the migration, proliferation and invasion of glioma cells in vivo and in vitro. Besides, PSMA3-AS1 bound to miR-302a-3p directly reduced the expression of miR-302a-3p, thus functioning as an endogenous sponge confirmed by luciferase reporter assay and bioinformatics analysis. PSMA3-AS1 knockdown remarkably enhanced the role of miR-302a-3p overexpression in cell behaviors in glioma. Moreover, these assays also confirmed that RAB22A was a target of miR-302a-3p. In this research, therefore, the PSMA3-AS1/miR-302a-3p/RAB22A pathway regulatory axis may be revealed in the pathogenesis of glioma, and PSMA3-AS1 can be used as an underlying target for the treatment and prognosis of glioma.
Highlights
As the most common solid tumor, glioma is the most common solid tumor in the central nervous system (CNS) [1,2]
Xia et al confirmed that FER1L4 adjusts the cycle and proliferation of glioma cells [9], Ni et al proposed that FoxD2-AS1 adjusts the PI3K/AKT signaling pathway as well as the miR-185-5P/HMGA2 axis, so as to accelerate the progression of glioma [10], and Yang et al found that long non-coding RNA (lncRNA) HERC2P2 is a tumor suppressor in glioma [11]
Expressed lncRNAs were identified according to the criteria of P 2
Summary
As the most common solid tumor, glioma is the most common solid tumor in the central nervous system (CNS) [1,2]. Multiple biological processes, including cell apoptosis, proliferation and metastasis in cancers are modulated by lncRNAs [6,7,8]. There are many lncRNAs related to glioma that have been reported. Xia et al confirmed that FER1L4 adjusts the cycle and proliferation of glioma cells [9], Ni et al proposed that FoxD2-AS1 adjusts the PI3K/AKT signaling pathway as well as the miR-185-5P/HMGA2 axis, so as to accelerate the progression of glioma [10], and Yang et al found that lncRNA HERC2P2 is a tumor suppressor in glioma [11]. PSMA3-AS1 (ENSG00000257621) is an lncRNA located on chromosome 14q23.1, and no reports examine the role of PSMA3-AS1 in glioma
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