Abstract
Osteosarcoma (OS) originates in the skeletal system and has a rising global incidence. Long Non-coding RNAs (lncRNAs) are key regulators of human cancers development and progression. However, their roles in the development of OS are not well understood. This research aimed to investigate the effect of a long non-coding RNA (lncRNA), MRUL, on OS and revealed its potential molecular mechanisms. The bioinformatics analysis demonstrated that lncRNA MRUL was involved in regulating nucleic acid-templated transcription, cellular macromolecule biosynthetic process, immune response, and inflammatory response. In this work, the expression of lncRNA MRUL was detected by quantitative real-time polymerase chain reaction (qRT-RCR) in both cancer tissues and cell lines. We found that lncRNA MRUL was up-regulated in cancer tissues and cell lines. Functional experiments showed that knockdown of lncRNA MRUL inhibited OS cell proliferation, and metastasis. At the same time, we found that lncRNA MRUL interacted with miR-125a-5p to suppress FUT4 expression. Moreover, inhibition of miR-125a-5p abrogated the biological roles of lncRNA MRUL knockdown on OS cell proliferation, migration, and invasion. In conclusion, these results demonstrated that OS-upregulated lncRNA MRUL promoted cell proliferation, and metastasis via negatively regulating miR-125a-5p, and imply that lncRNA MRUL may be a potential biomarker for OS.
Highlights
Long non-coding RNAs are linear RNAs that contain over 200 nucleotides in length (Chen et al, 2014)
The results showed that Long Non-coding RNAs (lncRNAs) MRUL positively co-expressing genes were associated
LncRNA MRUL negatively co-expressing genes were associated with the neutrophil activation involved in immune response (GO:0002283), cytokine-mediated signaling pathway (GO:0019221), antigen processing and presentation of exogenous peptide antigen via MHC class I (GO:0042590), inflammatory response (GO:0006954) (Figures 1C,D)
Summary
Long non-coding RNAs are linear RNAs that contain over 200 nucleotides in length (Chen et al, 2014) It accounts for about 95% of the total RNA, is widely distributed in the nucleus and cytoplasm and transcribed in eukaryotic cells (Gromesová et al, 2016). LncRNA played important roles in regulating gene expression in cells (Beermann et al, 2016; Fabbri et al, 2019). According to the research of Loewen et al (2014), lncRNA HOTAIR is highly expressed in lung cancer, associates with metastasis and poor prognosis. According to the research of Wang et al, in gastric cancer, Bioinformatics analysis demonstrated that MRUL might positively affect ABCB1 expression in an orientation- and position-independent manner (Wang et al, 2014)
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