Abstract
Background and objectiveLong non-coding RNAs can regulate tumorigenesis of various cancers. Dys-regulation of lncRNA-AFAP1-AS1 has not been studied in colorectal carcinoma (CRC). This study was to examine the function involvement of AFAP1-AS1 in tumor growth and metastasis of CRC.MethodsRelative expression of AFAP1-AS1 in CRC tissues and CRC cells lines was determined using quantitative real-time PCR (qRT-PCR). Functional involvement of AFAP1-AS1 in tumor proliferation and metastasis was evaluated in AFAP1-AS1-specific siRNA-treated CRC cells and in CRC cell xenograft. Expression of epithelial-mesenchymal transition (EMT)-related gene expression was determined using western blot.ResultsRelative expression of AFAP1-AS1 was significantly elevated in CRC tissues and CRC HCT116 and SW480 cell lines. AFAP1-AS1 knock-down suppressed SW480 cell proliferation, colony formation, migration and invasion. Also AFAP1-AS1 knock-down inhibited tumor metastasis-associated genes expression in terms of EMT. This carcinostatic action by AFAP1-AS1 knock-down was further confirmed by suppression of tumor formation and hepatic metastasis of CRC cells in nude mice.ConclusionlncRNA-AFAP1-AS1 knock-down exhibits antitumor effect on colorectal carcinoma in respects of suppression of cell proliferation and metastasis of cancer cells.
Highlights
Background and objectiveLong non-coding RNAs can regulate tumorigenesis of various cancers
Abnormal expression of actin filament-associated protein 1 (AFAP1)‐AS1 in colorectal carcinoma To determine the correlation between abnormal expression of AFAP1-AS1 and tumorigenesis of colorectal carcinoma, we examined relative expression of AFAP1AS1 in 15 clinical CRC tissues with paired para-carcinoma tissue as control
Relative expression of AFAP1AS1 was elevated in CRC cells lines of HCT116 and SW480 (Fig. 1b)
Summary
Long non-coding RNAs can regulate tumorigenesis of various cancers. Dys-regulation of lncRNA-AFAP1-AS1 has not been studied in colorectal carcinoma (CRC). This study was to examine the function involvement of AFAP1-AS1 in tumor growth and metastasis of CRC. Colorectal carcinoma (CRC) is the third cause of cancer incidence and death worldwide [1]. Number of CRC patients is increasing in China. Treatment methods have been improved, CRC still cannot be cure. Death occurs because of metastasis to other organ [2]. Long non-coding RNAs (lncRNAs) are kind of RNA molecule with a length of longer than 200 nucleotides and have no ability of coding protein [3]. As existing research results suggested that several lncRNAs are involved in transcriptional regulation, organization of
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