Abstract
A highly purified tRNA fraction isolated from sunflower mitochondria was used to probe a restriction fragment library of sunflower mitochondrial DNA. Approximately 20 different clones were detected. The 0.2-kbp HindIII insert of recombinant plasmid pHD500 was sequenced revealing the presence of a tRNA-His gene with 97% similarity to the equivalent of maize mitochondria and chloroplast. The single copy gene appears to be located at least 30 kbp distant from regions of high homology with cpDNA in the mitochondrial genome. This observation and other evidence (analysis of flanking regions and hybridization to cpDNA) suggest that the gene probably does not belong to a chloroplast insertion. The possible expression of the tRNA-His gene is discussed.
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