Abstract
Previous evidence has indicated that the testicular receptors for FSH reside on plasma membranes in seminiferous tubules, and that the Sertoli cell is a primary target of the hormone. Since the Sertoli cell is large and elaborately structured, it is important to know whether there is a regional distribution of receptors over its extensive surface. In the present study, the specific binding of 25I-labeled hFSH to seminiferous tubules of hypophysectomized rats was examined, utilizing autoradiography at both the light and electron microscope levels. Purified hFSH (LER-1575C) was iodinated to an average specific activity of 29 μCi/μg, and approximately 1 μg was administered intratesticularly in 40 day old rats which had been hypophysectomized 10 days earlier. In some cases, testosterone was administered after hypophysectomy in order to maintain spermatogenesis. Controls were of two types: 40 day old intact littermates in which endogenous FSH presumably occupied most of the receptors, and hypophysectomized rat...
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