Abstract
Summary Chloroplasts isolated from Brassica campestris (mustard) leaves were examined for superoxide dismutase activity. Cyanide sensitive (cuprozinc superoxide dismutase) and cyanide insensitive enzymes, were found predominantly in the stroma. Cyanide insensitive superoxide dismutase, as visualized by bands on Polyacrylamide gels, consisted of an iron containing isozyme and an unidentified protein which might be either a mangano superoxide dismutase or a mangano protein with superoxide dismutase activity. Grana were observed to contain less total superoxide dismutase than stroma. Incubation of chloroplasts with 0.8 M Tris HCl pH 8.0 resulted in a loss of photosystem 2 activity without concomitant superoxide dismutase inhibition. Prolonged treatment with Tris resulted in a loss of chloroplast membrane integrity followed by loss of stromal contents. Isolated photosystem 1 particles prepared either by detergent treatment or by sonication, showed little superoxide dismutase associated with these subchloroplast fragments.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
