Abstract
Ultrathin sections of fixed tissues were incubated with calf thymus terminal deoxynucleotidyl transferase and 3 H-dATP. The resulting incorporation of 3 H-dAMP into polydeoxynucleotide homopolymer was detected and localized by electronmicroscope autoradiography. Incorporated precursor is found to be TCA-insoluble and DNase-sensitive, and its localization indicates sites containing DNA. With this method, we demonstrate DNA in the nucleus, nucleolus, mitotic chromosomes and mitochondria.
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