Abstract
BackgroundThe role of long non-coding RNA (lncRNA) Minichromosome Maintenance Complex Component 3 Associated Protein (MCM3AP) Antisense RNA 1 (MCM3AP-AS1) has been analyzed in liver cancer. But its role in osteoarthritis (OA) is unknown. Through bioinformatics analysis, we predicted that MCM3AP-AS1 may interact with miR-142-3p, which is a major player in OA. This study aimed to investigate the roles of MCM3AP-AS1 in OA and to explore its interactions with microRNA miR-142-3p.MethodsDifferential expressions of MCM3AP-AS1 in OA patients and healthy participants were analyzed by performing quantitative PCR (qPCR). To analyze the relationship between MCM3AP-AS1 and miR-142-3p, human chondrocytes were transfected with MCM3AP-AS1 over-expression vector and miR-142-3p mimic. MCM3AP-AS1, miR-142-3p and high mobility group protein B1 (HMGB1) mRNA expression levels were measured by qPCR.ResultsWe found that MCM3AP-AS1 was up-regulated in OA. Bioinformatics analysis showed that MCM3AP-AS1 may interact with miR-142-3p, which can inhibit the apoptosis of chondrocytes. In addition, over-expression of MCM3AP-AS1 and miR-142-3p failed to affect the expression of each other. Instead, MCM3AP-AS1 over-expression led to up-regulated expressions of HMGB1, which is a target of miR-142-3p. Lipopolysaccharide (LPS) treatment led to the up-regulated expressions of MCM3AP-AS1 in chondrocytes. In cell apoptosis assay, MCM3AP-AS1 and HMGB1 over-expression led to increased apoptotic rate of chondrocytes. MiR-142-3p over-expression played an opposite role and attenuated the effects of MCM3AP-AS1 over-expression.ConclusionsMCM3AP-AS1 may regulate miR-142-3p/HMGB1 to promote LPS-induced chondrocyte apoptosis.
Highlights
The role of long non-coding RNA Minichromosome Maintenance Complex Component 3 Associated Protein (MCM3AP) Antisense RNA 1 (MCM3AP-AS1) has been analyzed in liver cancer
MCM3AP-AS1 was up-regulated in OA Expression levels of MCM3AP-AS1 in OA patients and healthy participants were measured by performing quantitative PCR (qPCR) was performed at 24 h post-transfection to confirm the over-expression of MCM3AP-AS1 and miR142-3p (Fig. 3a, p < 0.05)
MCM3AP-AS1 over-expression led to up-regulated high mobility group protein B1 (HMGB1) HMGB1 expression in chondrocytes with MCM3AP-AS1 and miR-142-3p over-expression at mRNA and protein levels were analyzed by qPCR (Fig. 4a) and western blot (Fig. 4b), respectively
Summary
The role of long non-coding RNA (lncRNA) Minichromosome Maintenance Complex Component 3 Associated Protein (MCM3AP) Antisense RNA 1 (MCM3AP-AS1) has been analyzed in liver cancer. Its role in osteoarthritis (OA) is unknown. We predicted that MCM3AP-AS1 may interact with miR-142-3p, which is a major player in OA. This study aimed to investigate the roles of MCM3AP-AS1 in OA and to explore its interactions with microRNA miR-142-3p. The development of OA damages joints and causes chronic pain and disability [2]. It is not lethal, the direct medical cost and the indirect productivity loss pose heavy economic burden on the modern society [3]. Occurrence of OA is predicted to be furtherly increased in near future, owing to the growing of aged population [5]. Treatment of OA is mainly focused on pain relief and disease
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