Abstract
BackgroundThe oncogenic role of the newly identified lncRNA LUADT1 has been revealed in lung adenocarcinoma. It was reported that LUADT1 plays a critical role in multiple human diseases. This study was carried out to investigate the role of LUADT1 in sepsis.MethodsSixty patients with sepsis and sixty healthy volunteers were recruited for this study. Plasma samples were collected from all participants. Human primary coronary artery endothelial cells were also used in this study. The expression of Pim-1, miR-195 and LUADT1 were detected by RT-qPCR. The interaction between miR-195 and LUADT1 was determined by overexpression experiments and luciferase activity assay. Cell apoptosis was detected by flow cytometry. The expression of apoptosis-related protein was detected by Western blotting.ResultsBioinformatics analysis revealed the potential interaction between LUADT1 and miR-195, which was confirmed by dual luciferase reporter assay. LUADT1 was downregulated in patients with sepsis. Moreover, LPS treatment downregulated the expression of LUADT1 in primary cardiac endothelial cells. Overexpression of LUADT1 and miR-195 did not affect the expression of each other in primary cardiac endothelial cells. Interestingly, overexpression of LUADT1 was found to upregulate the expression of Pim-1, a target of miR-195. In addition, it was found that overexpression of LUADT1 and Pim-1 reduced the enhancement effects of miR-195 on LPS-induced cardiac endothelial cell apoptosis.ConclusionIn summary, LUADT1 may protect cardiac endothelial cells against apoptosis in sepsis by regulating the miR-195/Pim-1 axis.
Highlights
Sepsis is a common and lethal clinical syndrome that occurs when severe systemic responses of body to infection, resulting in injury to its own tissues and organs (Cohen et al 2015)
LUADT1 was downregulated in patients with sepsis The expression of LUADT1 was detected in plasma from both sepsis patients (n = 60) and healthy controls (n = 60)
The results showed that the expression levels of LUADT1 were significantly and inversely correlated with the expression levels of miR-195 in sepsis patients (Fig. 1b, R2 = 0.0699, p < 0.05), while the expression levels of Pim-1 were positively correlated with that of LUADT1 (Fig. 1c, R2 = 0.1412, p < 0.05)
Summary
Sepsis is a common and lethal clinical syndrome that occurs when severe systemic responses of body to infection, resulting in injury to its own tissues and organs (Cohen et al 2015). In mice sepsis model, miR-195 is upregulated, and inhibition of miR-195 can reduce the injuries of multiple organs (Zheng et al 2015), suggesting that downregulation of miR-195 may be a potential therapeutic target for sepsis. LUADT1 is a newly identified oncogenic gene long non-coding RNA (lncRNA) in lung adenocarcinoma (Qiu et al 2015). Our bioinformatics analysis revealed the potential interaction between LUADT1 and miR-195. This study was carried out to investigate the potential interaction between LUADT1 and miR-195 in sepsis. The oncogenic role of the newly identified lncRNA LUADT1 has been revealed in lung adenocarcinoma. It was reported that LUADT1 plays a critical role in multiple human diseases. This study was carried out to investigate the role of LUADT1 in sepsis
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