Abstract
The development of skeletal muscle is a highly ordered and complex biological process. Increasing evidence has shown that noncoding RNAs, especially long-noncoding RNAs (lncRNAs) and microRNAs, play a vital role in the development of myogenic processes. In this study, we observed that lncMyoD regulates myogenesis and changes myofiber-type composition. miR-370-3p, which is directly targeted by lncMyoD, promoted myoblast proliferation and inhibited myoblast differentiation in the C2C12 cell line, which serves as a valuable model for studying muscle development. In addition, the inhibition of miR-370-3p promoted fast-twitch fiber transition. Further analysis indicated that acyl-Coenzyme A dehydrogenase, short/branched chain (ACADSB) is a target gene of miR-370-3p, which is also involved in myoblast differentiation and fiber-type transition. Furthermore, our data suggested that miR-370-3p was sponged by lncMyoD. In contrast with miR-370-3p, lncMyoD promoted fast-twitch fiber transition. Taken together, our results suggest that miR-370-3p regulates myoblast differentiation and muscle fiber transition and is sponged by lncMyoD.
Highlights
Mammalian skeletal muscle is a type of heterogeneous organ [1]
We found that lncMyoD sharply declined at 1 day to 3 weeks at the early stage of skeletal muscle development; it was significantly upregulated at 3–4 weeks when skeletal muscle was differentiated into mature muscle tissue (Figure 1A)
This result suggests that lncMyoD may participate in skeletal muscle development, and we observed a dynamic process in skeletal muscle development
Summary
Mammalian skeletal muscle is a type of heterogeneous organ [1]. An increasing number of studies have shown that skeletal muscle serves multiple functions in addition to movement, including secreting regulatory factors for crosstalk with distant organs and executing metabolic organ function [2,3]. Skeletal muscle dysfunction may cause human diseases and damage individual health [4,5,6]. Muscle progenitor cells undergo a highly coordinated process during myogenesis, including myoblast proliferation, differentiation, and cell fusion into multinucleated myotubes. Growing evidence indicates that fiber-type composition is closely associated with livestock meat quality [7,8,9]. Uncovering the mechanism of myoblast differentiation and changes in fiber-type composition is necessary for both humans and livestock
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