List of field crop varieties registered in the Czech Republic in 2025

The onion (Allium cepa L.) constitutes an important part of the human diet and is of economic importance on the vegetable market. Methods that allow unambiguous differentiation of cultivars and breeding lines are required for market control and the protection of plant breeders' rights. DNA analyses, such as single sequence repeat polymorphism (SSRs), represent such a tool. Based on the evaluation of polymorphism in 21 SSR loci, we established a panel of 15 easy to score SSR markers that differentiated 16 commercial onion cultivars in the Czech Republic. The polymorphism ranged from 2-3 alleles per locus, indicating a low level of diversity among the cultivars. The panel can also be used to evaluate genetic resources.

Quality of green fodder from a semi-natural sward consisting of Festuca rubra, Taraxacum officinale, Dactylis glomerata, Trisetum flavescens, Poa ssp., Agrostis stolonifera and Phleum pratense as dominant species, situated in the Bohemian-Moravian Highlands (Czech Republic) was studied in the months of the main forage utilization (November, December and January). Main usage in the winter months was preceded by usage in June, July and August (preparatory cut). The sward was fertilized in the first half of August with 50 kg N.ha-1. Qualitative characteristics studied in 2000/2001, 2001/2002 and 2002/2003 were N-substances and NEL. Sward quality was decreasing from November to January with the date of utilization exhibiting a highly significant effect (α < 0.01) on the NEL content in all three years of monitoring and on the content of N-substances in the first two years of monitoring. Effect of the preparatory cut on the contents of N-substances and NEL was significant (α < 0.05) in all three years of monitoring and in the first two years of monitoring, respectively. In November, the contents of N-substances and NEL were higher in variants with the preparatory cut made in August than in variants with the preparatory cut made in June or July. The effect of the date of preparatory cut on the contents of N-substances and NEL in December and January was depending on climatic conditions in the given year.

More than 40 kinds of vegetable and melons are grown in Uzbekistan. From the main vegetable crop varieties tomatoes, cucumbers, onions, carrots, cabbage, beets, sweet and spicy peppers, eggplant, greens, watermelon, melon, pumpkin are planted in large fields. In recent years, export-oriented tours such as rose cabbage, Chinese cabbage, broccoli, kohlrabi, daikon from less common vegetable crops are gaining ground. The soil-climatic conditions of Uzbekistan are rich in vitamins, environmentally friendly, very suitable for the cultivation of export-oriented products. The length of the days of the groom in our country and the high temperature increase the amount of biologically active substances and vitamins contained in the products of vegetables, melons. This is increasing the demand for our products. Today, tomatoes, cucumbers, onions, carrots, cabbage, soup beets, sweet and spicy peppers, eggplant, Redis, greens, cauliflower, broccoli, kohlrabi, iceberg salad, watermelon, melon products are exported from vegetable crops. From Uzbekistan in January-July, 2020 amounted to 147.1 million of US dollars, vegetables were exported. In order to increase the export potential, more than 60 varieties of vegetables and melons have been created at the Research Institute of vegetable, melons and potatoes in recent years. The main style of our selection works is separated from the collection the forms of motherhood and chattering works are conducted. Descendants of hybrids are divided on the basis of tests, prospective forms are selected. Perspective lines are divided into perspectives for a test the new variety in standard variety. Then, small and large varieties are tested with zoned varieties in Test nurseries. At the same time, primary seeding of perspective lines is organized. Prospects based on the results of the competition and production will be submitted to the state variety test Commission. On the basis of this method, a selection of tomato, cucumber, onion, watermelon and melon crops is carried out. This article describes the results of the selection work obtained in recent years.

Complex aspects of climate change impacts on the cultivation of perennial energy crops in the Czech Republic

Mycotoxins are naturally occurring secondary metabolites produced by several toxigenic microscopic fungi on a variety of crops, especially cereal grains and further foodstuffs. Series of experimental research projects on the determination of mycotoxins (aflatoxins, cyclopiazonic acid, ochratoxin A, patulin, deoxynivalenol, fumonisin B1, T-2 toxin, zearalenone, sterigmatocystin, alternaria toxins) in several foods were realized in the National Reference Centre for Microscopic Fungi and Mycotoxins in the 90(th) years. The aim of our work was an estimation of dietary exposure to mycotoxins and risk assessment. The method of a solid phase extraction (SPE), liquid - liquid extraction and immunoaffinity chromatography (f. e. R-Biopharm, VICAM) were used to elaborate for sample analyses of mycotoxins in our projects. The mycotoxins were detected most frequently by chromatographic methods (HPTLC, HPLC, GC) and immunochemical methods (ELISA). Average dietary exposure has been calculated by multiplying of concentration data for specific foods with their consumption rates per 1 kg of b. w. per day. The estimation of the dietary exposure dose of mycotoxins for the Czech population is presented.

Transcriptome and metabolome integrated analysis reveals the wax biosynthesis mechanism of Allium cepa L.

There is increasing interest in the use of amino acid-based biostimulant products due to their reported abilities to improve a number of quality characteristics in a variety of specialty crops. However, when it comes to the foliar application of amino acids to turfgrass, there are still many basic questions about their uptake forms and incorporation into cellular metabolism. In this study, we shed light on the fate of amino acids exogenously applied to turfgrass foliage through a series of time-course, isotopic-labeling studies in creeping bentgrass (Agrostis stolonifera L.) leaves. Using both 15N-labeled and 15N,13C double-labeled L-glutamate applied exogenously to creeping bentgrass foliage, we measured the uptake of glutamate and its integration into γ-aminobutyric acid (GABA) and L-proline, two amino acids with known roles in plant stress adaptation. Our results demonstrate that glutamate is rapidly absorbed into creeping bentgrass foliage and that it is utilized to produce GABA and proline. Based on the labeling patterns observed in the endogenous pools of glutamate/glutamine, GABA, and the proline from applied glutamate-[13C515N1], we can further conclude that glutamate is predominantly taken up intact and that mineralization into other forms of nitrogen is a minor fate. Taken together, the collective findings of this study provide evidence that amino acids exogenously applied to turfgrass foliage can be rapidly absorbed, and serve as stable sources of precursor molecules to be integrated into the metabolism of the plant.

This work describes anovel partitivirus genome assembled from RNA-seq data generated from onion tissue from fields in Brazil. Anew partitivirus genome composed of three dsRNAs, which was closely related to arhar cryptic virus 1, was assembled from Allium cepa samples from Brazil. The genomic sequences were also identified from available transcriptomic datasets of onion samples from China, Czech Republic, India, South Korea and USA. According to the species demarcation in the Partitiviridae family, the new virus was classified into the genus Deltapartitivirus with the suggested name of allium deltapartitivirus. This is the first report of the occurrence of acryptic virus in plants of the genus Allium, and therefore, this work contributes to the understanding of the genetic diversity of partitiviruses that infect the genus Allium. Keywords: Allium sp.; high-throughput sequencing; partitiviruses.

The use of diverse genetic resources to breed improved crop varieties has been a key driver of agricultural productivity improvements in the past century. At the same time, the adoption of modern varieties has contributed to substantial loss of traditional varieties. In this analysis, we estimate the social value provided by several proposed crop diversity conservation programs to be carried out by the Czech genebank system. We use a double-bounded dichotomous choice model to estimate the willingness-to-pay (WTP) for conserving additional crop varieties in the genebank for ten years using data collected through an online contingent valuation survey administered to a sample representative of the general Czech population (1037 respondents) and a smaller sub-sample representative of the agricultural region of South Moravia (500 respondents). Mean WTP was found to be about $9 for both the Czech and S. Moravian sub-samples, corresponding to country-wide benefits of ~$68 million. These benefits increase by 6–7% for every ten varieties conserved, implying total welfare benefits of ~$84 million for a program conserving the maximum number of 35 additional crop varieties offered in the experiment. The study illustrates an empirical approach of potential value for policymakers responsible for determining funding levels for genetic resource conservation.

In some plants, sucrose: sucrose 1-fructosyltransferase (1-SST) is the first irreversible key enzyme in fructan biosynthesis. Studies have shown that fructan accumulation enhances abiotic stress tolerance of plants. To investigate the role of 1-SST in drought stress responses, a total of 37 cotton plants expressing a 1-SST gene from Allium cepa were developed by Agrobacterium-mediated transformation. Under drought stress in the field, compared with wild-type, ectopic expression of Ac1-SST in cotton resulted in significantly higher soluble sugars (especially 1-kestose), proline and relative water contents, as well as decreased malondialdehyde content, which contributed to maintaining intracellular osmoregulation and reducing membrane damage. In addition, ectopic expression of Ac1-SST in cotton significantly improved the photosynthesis rate, performance of PSII (including Pn, Fv/Fm, WUE, ΦPSII, and PItotal) and plant growth under drought stress. Furthermore, compared with the wild-type, under the droughted field, the yield loss per square meter of transgenic cotton was reduced by an average of 20.9% over two consecutive years. Our results indicate that the Ac1-SST gene can be used to improve drought tolerance and yield of cotton varieties, and might also be a promising drought-resistant gene for improving other crop varieties.

Two ectoparasite powdery mildew species <i>Golovinomyces cichoracearum</i> (<i>Gc</i>) and <i>Podosphaera xanthii</i> (<i>Px</i>) occurring on cucurbits differ, besides other characteristics, by specific ecologic requirements. While <i>Px</i> is common in subtropical and tropical areas and greenhouse crops, <i>Gc</i> occurs more frequently in temperate and cooler areas under field conditions. Their presence on cucurbit field crops (<i>Cucurbita pepo</i>, <i>C. maxima</i>, <i>Cucumis sativus</i>) was monitored in the Czechoslovakia (1979–1980) and in the Czech Republic (1995–2007). Their identification was carried out by microscopic observation of the morphological characteristics of the dry conidia on 1527 leaf samples. Data on air temperature in 1979–2007 were provided by the Czech Hydrometeorological Institute. In 1979–1980 <i>Gc</i> was identified in 86.0% of samples, <i>Px</i> in 14.0% samples, there was no mixed infection; prevalence of <i>Px</i> was recorded in South Slovakia and South Moravia, and on crops under cover. Since 1995 species <i>Px</i> was recorded each year on field crops in different locations of Bohemia and Moravia, usually in mixed infection with <i>Gc</i>. The average year temperature of 8.1°C for period 1992–2007 was higher than corresponding value of 7.4°C in 1979–1983. Similarly, average temperature in vegetation season of 16.2°C in 1992–2007 was higher than corresponding value of 15.7°C in 1979–1983. The higher air temperature can positively influence spreading of <i>Px</i> in the Czech Republic. Climate variability and effect of their changes are discussed in relationship to the geographic distribution and geographic patterns of cucurbit powdery mildews.

Goal. To investigate the effectiveness of fungicidal control of the most common onion diseases during the growing season.
 Methods. Informational and analytical (collection of materials and analysis of literary sources), field research (setting up experiments, carrying out treatments and records of plant damage by diseases, monitoring the development of plants, harvesting), phytopathological (determination and identification of pathogens), mathematical and statistical (processing of results studies). Experiments were conducted on different varieties and hybrids using modern fungicides with different active substances.
 Results. During the years of research, onion crops were dominated by mycoses, namely, peronosporosis, alternaria, stemphylliosis, and fusarium wilt. Downy mildew and leaf spots were observed on all varieties and hybrids. The investigated fungicides most effectively controlled the development of downy mildew of onions. Their efficiency during the growing season averaged 55.1—100%, depending on the drug, the degree of disease development and the variety. The highest effectiveness against downy mildew of onions was provided by fungicides Fandango 200 ES, KE (fluoxystrobin, 100 g/l + proteoconazole, 100 g/l) at the rate of 1.25 l/ ha and Signum VG (boskalid, 267 g/ kg + pyraclostrobin, 67 g/ kg) — 1.5 kg/ ha. Fungicides were 45.8—89.1% effective against leaf spots (alternaria and stemphyllosis), 57.9—70.5% against fusarium wilt. Treatments with fungicides, due to the reduction of plant damage by diseases, contributed to the increase in onion yield by 1.3—6.9 t/ha, depending on the variety and preparation. Among the studied varieties, the highest yield was obtained on the Medusa variety (40.7 t/ha), among fungicides, the highest yield increase was provided by Fandango 200 ES, KE fungicide (1.25 l/ha).
 Conclusions. Fungicides effectively controlled the main mycoses of onion (peronosporosis, alternariosis, stemphyllosis and fusarium wilt) during the growing season. The effectiveness of fungicides against onion diseases was 45.8—100%, depending on the type of disease, drug and crop variety. The highest protective effect of fungicides was provided against downy mildew of onions. The yield of the researched varieties and hybrids increased by an average of 5—25% due to the reduction of plant damage by diseases. The highest technical efficiency against identified diseases and increase in onion yield was obtained by using Fandango 200 ES, KE fungicide with a consumption rate of 1.25 l/ha.

A genetic marker system based on the S1 Short Interspersed Elements (SINEs) in the important commercial crop, oilseed rape ( Brassica napus L.) has been developed. SINEs provided a successful multilocus, dominant marker system that was capable of clearly delineating winter- and spring-type crop varieties. Sixteen of 20 varieties tested showed unique profiles from the 17 polymorphic SINE markers generated. The 3' or 5' flank region of nine SINE markers were cloned, and DNA was sequenced. In addition, one putative pre-transposition SINE allele was cloned and sequenced. Two SINE flanking sequences were used to design real-time PCR assays. These quantitative SINE assays were applied to study the genetic structure of eight fields of oilseed rape crops. Studied fields were more genetically diverse than expected for the chosen loci (mean H T = 0.23). The spatial distribution of SINE marker frequencies was highly structured in some fields, suggesting locations of volunteer impurities within the crop. In one case, the assay identified a mislabeling of the crop variety. SINE markers were a useful tool for crop genetics, phylogenetics, variety identification, and purity analysis. The use and further application of quantitative, real-time PCR markers are discussed.

Cytogenetic toxicity from pesticide and trace element mixtures in soils used for conventional and organic crops of Allium cepa L.

Naturally-occurring wetlands perform such functions as flood control, pollution filtration, nutrient recycling, sediment accretion, groundwater recharge and water supply, erosion control, and plant and wildlife preservation. A large concentration of wetlands is located in Eastern Europe. A significant amount of Eastern European wetlands has been converted to agricultural use in the past, and remaining wetlands are subject to agricultural drainage. Drained wetlands are used as prime agriculture lands for a variety of food crops. Other agricultural uses of wetlands range from growing Phragmites australis (common reed) for thatch and livestock feed, to collecting peat for heating and cooking fuel. Altered hydrologic regimes due to global climate change could further exacerbate encroachment of agricultural land use into wetlands. The vulnerability and adaptation studies of the U.S. Country Studies Program are used to analyze where climate change impacts to agriculture may likewise impact wetland areas. Scenarios indicate higher temperatures and greater evapotranspiration altering the hydrologic regime such that freshwater wetlands are potentially vulnerable in Bulgaria, Czech Republic, and Russia, and that coastal wetlands are at risk in Estonia. Runoff is identified as a key hydrological parameter affecting wetland function. Since wetland losses may increase as a result of climate-change-induced impacts to agriculture, precautionary management options are reviewed, such as establishing buffer areas, promoting sustainable uses of wetlands, and restoration of farmed or mined wetland areas. These options may reduce the extent of negative agricultural impacts on wetlands due to global climate change.