Liquid chromatography–tandem mass spectrometry method for the determination of tranexamic acid in human plasma

Abstract

A new method for the determination of tranexamic acid (TA) in human plasma using high performance liquid chromatography with tandem mass spectrometric detection was described. TA and the internal standard, methyldopa, was extracted from a 200 μl plasma sample by a one-step deproteination using perchloric acid. Chromatographic separation was performed on an Xtrra™ MS C 18 Column ( 2.1 mm×100 mm, 3.5 μm) with the mobile phase consisting of 10% acetonitrile in 2 mM ammonium acetate buffer (pH 3.5) at a flow rate of 0.15 ml/min. The total run time was 5 min for each sample. Detection and quantitation was performed by the mass spectrometer using the multiple reaction monitoring of the precursor-product ion pair m/ z 158 → 95 for TA and m/ z 212 → 166 for methyldopa, respectively. The method was linear over the concentration range of 0.02–10.00 μg/ml with lower limit of quantification of 0.02 μg/ml for TA. The intra- and inter-day precision was less than 11% and accuracy ranged –10.88 to 11.35% at the TA concentrations tested. The present method provides a relatively simple and sensitive assay with short turn-around time. The method has been successfully applied to a clinical pharmacokinetic study of TA in 12 healthy subjects.