Abstract
This paper reviews high-performance liquid chromatographic (HPLC) methods with chemiluminescence (CL) detection for the analysis of biologically important fatty acids. A CL detection method dose not need an exciting light, which can eliminate any scattering of light source. Therefore, the method is highly sensitive owing to a large signal-to-noise ratio (S/N). Further, the method has advantages over other detection method for HPLC—high selectivity, wide dynamic range, and relatively simple instrumentation. Through these advantages, the HPLC with CL detection has increasing importance in the areas of clinical and biomedical analyses. In this review, CL dereivatization reagents for fatty acids and their utilization to the CL detection methods for HPLC were discussed in detail.
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