Abstract
The residues of [ 3 H]-17-methyltestosterone (17MT) in rainbow trout after a single intragastric dose (200 μg, 0.37 MBq) were investigated. The metabolites were extracted from liver and carcass with chloroform—methanol (2 + 1, v/v). After enzymatic hydrolysis of the glucuronides, unconjugated steroids and aglycones were chromatographed by reversed-phase liquid chromatography. Each labelled fraction was collected and analysed by gas chromatography—mass spectrometry. After 24 h, the liver contained 4% of the administered dose. In the hepatic unconjugated and aglycone fractions the major metabolite was an isomer of 17α-methylandrostane-3ξ,16ξ,17β-triol. 7α-Hydroxy-17α-methylandrostane-3α,17β-diol was also observed in the unconjugated fraction. In addition to these metabolites, unchanged 17MT, 17α-methyl-5β-androstane-3α,17β-diol and 17β-hydroxyandrosta4,6-dien-3-one were identified in the unconjugated fraction extracted from carcass samples. After 72 h, 7α-hydroxy-17α-methylandrostane-3α,17β-diol persisted to a significant extent in carcass samples whereas no trace of 17MT was detected. This metabolite could therefore be used as an indicator of the administration of 17MT in fish.
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