Abstract
Newly developed uses of fat soluble pigments which change in color depending upon the quantity of fatty acids, and of nutrient agar media containing emulsified milk fat are among the methods for the measurement of the lipolitic activities of bacteria for milk fat.Studies on lipolitic bacteria in raw milk by the use of the Victoria blue base-margarine fat nutrient agar medium which had been deviced recently by E. L. Crossley, resulted in isolating Micrococcus, Alcaligenes, Pseudomonas and other Gram-negative bacilli.Use of Hugh-Leifson medium and check of pigment producing ability by cytochrome oxidase test seemed helpful for characterization of Gram-negative bacilli.
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