Abstract
We present a method for giant lipid vesicle shape analysis that combines manually guided large-scale video microscopy and computer vision algorithms to enable analyzing vesicle populations. The method retains the benefits of light microscopy and enables non-destructive analysis of vesicles from suspensions containing up to several thousands of lipid vesicles (1–50 µm in diameter). For each sample, image analysis was employed to extract data on vesicle quantity and size distributions of their projected diameters and isoperimetric quotients (measure of contour roundness). This process enables a comparison of samples from the same population over time, or the comparison of a treated population to a control. Although vesicles in suspensions are heterogeneous in sizes and shapes and have distinctively non-homogeneous distribution throughout the suspension, this method allows for the capture and analysis of repeatable vesicle samples that are representative of the population inspected.
Highlights
Lipid vesicle studying techniques range from visual investigation of individual vesicles to population studies of vesicles in suspension
At multiple times, the vesicles were sampled by recording videomicroscopy sequences that were later analyzed using computer vision algorithms to count the vesicles and calculate their sizes and isoperimetric quotients
We present a method for analyzing lipid vesicles that allows us to nondestructively conduct shape and size analysis of thousands of vesicles and simultaneously allows for visual inspection of individual ones
Summary
Lipid vesicle studying techniques range from visual investigation of individual vesicles to population studies of vesicles in suspension. At multiple times (exact numbers in Table 1), the vesicles were sampled by recording videomicroscopy sequences that were later analyzed using computer vision algorithms to count the vesicles and calculate their sizes and isoperimetric quotients.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
