Abstract
ABSTRACT The aim of this research was to evaluate the effect of partial substitution of vitamin E (VE) by grape seed extract (GSE) on glutathione peroxidase activity (GPxA), nitric oxide (NO) concentration and lipid peroxidation [malondialdehyde (MDA) concentration] in the plasma, lungs, heart and liver, and live performance of broilers raised at 2278 m of altitude. One-d-old Ross 308 male chickens (n = 420) were randomly distributed into three treatments: Control-AL (basal diet containing 40 IU of VE and fed ad libitum; AL), Control-FR (basal diet and feed restriction; FR) and GSE-AL [basal diet containing 10 mg of GSE (equivalent to 30 IU VE) plus 10 IU of VE and fed AL]. The data were analyzed using one-way analysis of variance. Glutathione peroxidase activity, and NO and MDA concentrations in the plasma were evaluated on d 46. On d 47, MDA was evaluated in the lungs, heart and liver. Live performance parameters were recorded weekly. There were no significant differences (p>0.05) in live performance and NO concentration among treatments. Birds from the GSE-AL treatment presented the lowest (p<0.05) GPxA, the highest (p<0.05) MDA concentration in the plasma, heart and liver, and intermediate MDA concentration in the lungs. Our results suggest that GSE may partially replace VE in broiler diets without impairment of live performance. However, further research is required to determine the optimal level of dietary GSE inclusion to reduce lipid peroxidation in the plasma, lungs, heart, and liver of broilers raised at 2278 m of altitude.
Highlights
Ascites syndrome (AS) is a metabolic disorder (Bautista-Ortega & RuizFeria, 2010) that mostly occurs in fast-growing chickens (Arab et al, 2006)
There were no significant differences in Body weight gain (BWG), feed intake (FI), or feed conversion ratio (FCR) among treatments (Table 1)
The results of this research showed that grape seed extract (GSE) may partially replace vitamin E (VE) in broiler diets with no impairment of their live performance
Summary
Ascites syndrome (AS) is a metabolic disorder (Bautista-Ortega & RuizFeria, 2010) that mostly occurs in fast-growing chickens (Arab et al, 2006). Poor ventilation (Julian, 2000), low temperature (Fathi et al, 2011), and ad libitum feeding (Rodríguez-Ortega et al, 2014) are predisposing factors for AS. High altitude reduces the partial pressure of oxygen (PO2) in the inspired air (hypoxia, Wideman et al, 2013), which in turn produces hypoxic broilers (Ruiz-Feria & Wideman, 2001). Bakonyi & Radak (2004) reported that exposure to high altitude increases the formation of reactive oxygen species (ROS) and reactive nitrogen species, and ROS cause lipid peroxidation in heart and liver of broilers (Díaz-Cruz et al, 1996). Rodríguez-Ortega et al (2014) observed that ad libitum feeding decreases antioxidant activity in lungs, heart, and liver of broilers The central etiology of AS is a hypoxemic condition (hypoxemia refers to blood in the systemic arteries that is under saturated with oxygen; Wideman et al, 2013) resulting from an imbalance between demand and supply of oxygen (Kalmar et al, 2013). Bakonyi & Radak (2004) reported that exposure to high altitude increases the formation of reactive oxygen species (ROS) and reactive nitrogen species, and ROS cause lipid peroxidation in heart and liver of broilers (Díaz-Cruz et al, 1996). Bottje et al (1995) reported that oxidative stress is associated with AS. Rodríguez-Ortega et al (2014) observed that ad libitum feeding decreases antioxidant activity in lungs, heart, and liver of broilers
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
