Abstract
The relationship between lipid peroxidation, glutathione (GSH) content, and CCl 4-induced toxicity was investigated in rat hepatocytes isolated by a collagenase-perfusion technique. Two chemical initiators of lipid peroxidation, ferric ions complexed with adenosine diphosphate ( ADP Fe 3+ ) and diethyl maleate, were studied for comparison. CCl 4 caused a reduction of intracellular K + and release of alanine aminotransferase (ALT) into the medium, but no evidence of lipid peroxidation, as measured by the absorbance of thiobarbituric acid (TBA)-reacting materials and lipid-extract diene conjugation. ADP Fe 3+ caused lipid peroxidation, but only a small loss of K +. Diethyl maleate caused a greater amount of lipid peroxidation and cell damage than did ADP Fe 3+ . Neither response appeared to be related to the GSH content, which was reduced by diethyl maleate, but not by ADP Fe 3+ , and by CCl 4 only at the highest dose. The results suggest that lipid peroxidation is not a requisite step in CCl 4-induced toxicity in isolated hepatocytes.
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