Abstract
Research into the phase separation of coexisting liquid phases in mixed phospholipid/sterol monolayer systems is an important experimental approach to understand the lateral inhomogeneities or “lipid rafts” within lipid membranes. We present measurements of line tensions between immiscible phases in mixed monolayer systems of phospholipids and the cholesterol analog 25-hydroxycholesterol. This hydroxycholesterol is an interesting modulation of the cholesterol structure for both its implicated pathological effect on the plasma cell membrane as well as its unique phase diagram. In addition to these experimental studies we will also discuss ongoing work to improve our line tension measuring tools. Model-convolution microscopy is a technique that can be used to assess the effectiveness of image processing routines by testing them against experimenter determined parameters. Here a theoretical model is used to generate the underlying structure of an image and this is convolved with the point spread function of light. We will also present results obtained using this technique to study the importance and necessity of the incompressibility constraint in the Fourier analysis of lipid domains.
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