Abstract
Abstract 1. 1. Nuclear membrane fractions were prepared from rat-liver nuclei by treatment with deoxyribonuclease and MgCl2. 2. 2. The lipid distribution and fatty acid composition of total lipids and individual phospholipids was determined. 3. 3. The ratio of protein to total lipid was 2.96. Phospholipids accounted for 62% of the total lipid fraction. The remainder was accounted for as neutral lipids. Non-phosphorus-containing polar lipids were not detected. Based on lipid phosphorus distribution the major phospholipids were phosphatidylcholine (55%), phosphatidylethanolamine (23%), phosphatidylinositol (8%), phosphatidylserine (6%) and sphingomyelin (6%). Cholesterol and free fatty acids were the major constituents of the neutral lipid fraction, accounting for t6 and t3%, respectively, of the total membrane lipids. 4. 4. Phosphatidylcholine was the least saturated and sphingomyelin the most saturated of the major phospholipids. Eighteen-carbon chain-length fatty acids accounted for 70% of the acyl residues of the total lipid fraction. The palmitic acid to oleic acid ratio of the total lipid fraction was 0.55.
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