Abstract
Incubation of an enzyme preparation of Rhizobium meliloti with labeled uridine diphosphate glucose led to the formation of radioactive substances soluble in organic solvents. These substances are probably polyprenyl diphosphate saccharides. They behaved like these on treatment with ammonia or with hot phenol and were decomposed by heating for 10 min at pH 2 yielding a mono- and a disaccharide. The monosaccharide was identified as galactose by paper chromatography. The disaccharide gave glucose and galactose by acid hydrolysis. Following reduction with borohydride it yielded glucose and galactitol. After treatment with periodate followed by paper chromatography only galactose was detectable. The disaccharide was hydrolyzed by β- but not by α-glucosidase. Therefore the disaccharide is glucosyl β1-3-galactose.
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