Abstract
Publisher Summary This chapter elaborates on lipase, which hydrolyses emulsified triglycerides of the long-chain fatty acids. The concentration of lipase in normal serum is low. A rise in the lipase content of serum is observed in acute and chronic renal disease. In cases of pancreatitis, no lipase activity can be detected in urine. The number of fatty acid ester bonds hydrolysed per unit time, as determined by the amount of NaOH required to maintain a constant pH, is a measure of the lipase activity. The pH optimum for pancreatic lipase is pH 8.6–9.0. As the active concentration of the substrate depends on the water–oil interface, the substrate must be prepared as an emulsion, which will be stable for the period of the assay. With measurements in the lower range of the sensitivity of the method, there is a slight alkaline hydrolysis of the gum arabic, which can lead to errors because it is not linear in the first 4 minutes. The preneutralization of the olive oil is absolutely necessary, because the presence of free oleic acid affects the uniformity of the emulsion. For a large number of assays, automation by inclusion of a pH-stat is an advantage.
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