Lipase in Pancreas and Intestinal Contents of Chickens Fed Heated and Raw Soybean Diets

Abstract

PREVIOUS work has shown that ingestion of raw soybean (RS) diets caused greater decreases in proteases (Lepkovsky et al., 1965) and amylase (Lepkovsky et al., 1966) in the chicken pancreas than did ingestion of heated soybean (HS) diets. Those decreases were accompanied by larger amounts of amylase in the intestinal contents. The amounts of proteases in the intestinal contents in the chickens fed RS diet were masked by the complexing of trypsin with the trypsin inhibitors in the RS.In the study reported here, lipase activity was measured in: 1) pancreas and intestinal and cecal contents of intact chickens; and 2) intestinal contents, and intestinal effluent collected through ileostomies, of chickens fed HS and RS diets.EXPERIMENTAL PROCEDUREExperiment 1. Intact chickens. Five-week-old Single Comb White Leghorn female chickens weighing 400 to 500 gm. were fed HS or RS diets*, with and without methionine supplement, for 6 weeks. At autopsy,…