Abstract
Faba bean (Vicia faba L.), a partially allogamous species, is rich in protein. Condensed tannins limit the use of faba beans as food and feed. Two recessive genes, zt-1 and zt-2, control the zero tannin content in faba bean and promote a white flower phenotype. To determine the inheritance and develop a linkage map for the zt-1 gene in the faba bean germplasm M3290, F2 and F3 progenies were derived from the purple flower and high tannin content genotypes Qinghai12 and zt-1 line M3290, respectively. Genetic analysis verified a single recessive gene for zero tannin content and flower colour. In total, 596 SSR markers and 100 ISSR markers were used to test the polymorphisms between the parents and bulks for the contrasting flower colour via Bulked Segregant Analysis (BSA). Subsequently, six SSR markers and seven ISSR markers were used to genotype the entire 413 F2 population. Linkage analysis showed that the zt-1 gene was closely linked to the SSR markers SSR84 and M78, with genetic distances of 2.9 and 5.8 cM, respectively. The two flanked SSR markers were used to test 34 faba bean genotypes with different flower colours. The closely linked SSR marker SSR84 predicted the zt-1 genotypes with absolute accuracy. The results from the marker-assisted selection (MAS) from this study could provide a solid foundation for further faba bean breeding programmes.
Highlights
Faba bean (Vicia faba L.), one of the most important temperate food crops, is widely grown for human consumption in China, Ethiopia, Egypt and the Andean States of South America and for livestock feed in Europe and Australia [1]
The objective of this study was to (1) construct a linkage map of the temporary named gene zt-1 the controls the zero-tannin trait in the M3290 variety and (2) identify closely linked markers that could be useful for marker-assisted selection (MAS) in faba bean and further cloning of the gene
We tested the elite faba bean germplasms with the closely linked markers and the results provided a sound basis for further MAS
Summary
Faba bean (Vicia faba L.), one of the most important temperate food crops, is widely grown for human consumption in China, Ethiopia, Egypt and the Andean States of South America and for livestock feed in Europe and Australia [1]. The average faba bean cultivation area is close to 2.5 million hectares annually, which ranks fourth among cool-season crops [2]. It has been demonstrated that growing faba bean is the most effective strategy for managing soil fertility through crop rotation, which contributes to sustainable agriculture [3]. Faba bean seeds together with other relative beans, have high nutritional values as they are excellent sources of protein, carbohydrates, minerals and fibre [4]. Faba bean suffer from both biotic and abiotic factors that constrain their productivity and digestibility. Previous studies have demonstrated that condensed tannins are responsible for low-protein seeds and may Agronomy 2018, 8, 80; doi:10.3390/agronomy8060080 www.mdpi.com/journal/agronomy
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
