Abstract
BackgroundAccumulating evidence has revealed the critical role of long non-coding RNAs (lncRNAs) in cellular processes during tumor progression. As documented in cancer-related literatures, LINC00992 expression is associated with cancer progression, whereas its function in tumors including prostate cancer has not been characterized yet.MethodsData from GEPIA database suggested LINC00992 expression in prostate cancer tissues. The expression levels of RNAs were monitored via qRT-PCR. Western blot evaluated the levels of proteins. The proliferation, apoptosis and migration of prostate cancer cells were assessed by CCK-8, EdU, TUNEL, Transwell and wound healing assays. Luciferase reporter, RNA pull down and RIP assays were applied to detect the interplays among LINC00992, miR-3935 and GOLM1.ResultsElevated levels of LINC00992 and GOLM1 were detected in prostate cancer tissues and cells. LINC00992 exerted facilitating functions in prostate cancer cell proliferation and migration. Mechanically, LINC00992 interacted with and negatively regulated miR-3935 to elevate GOLM1 expression in prostate cancer cells. In addition, the in vitro suppressive effect of silenced LINC00992 on prostate cancer cell proliferation and migration was reversed by GOLM1 upregulation. Likewise, LINC00992 depletion restrained tumor growth in vivo was offset by enhanced GOLM1 expression.ConclusionsLINC00992 competitively bound with miR-3935 to elevate GOLM1 expression and therefore facilitate the oncogenic phenotypes of prostate cancer cells, implying a potential LINC00992-targeted therapy for prostate cancer.
Highlights
Accumulating evidence has revealed the critical role of long non-coding RNAs in cellular processes during tumor progression
LINC00992 is overexpressed in prostate cancer and regulates cell proliferation, apoptosis and migration LINC00992 expression pattern in prostate cancer was acquired from online GEPIA database
After detecting LINC00992 expression in tissue samples obtained from patients with prostate cancer, we observed that LINC00992 expression was higher in prostate cancer tissues than that in peri-tumor tissues (Figure S1A)
Summary
Accumulating evidence has revealed the critical role of long non-coding RNAs (lncRNAs) in cellular processes during tumor progression. As documented in cancer-related literatures, LINC00992 expression is associated with cancer progression, whereas its function in tumors including prostate cancer has not been characterized yet. The widely studied long noncoding RNAs (lncRNAs) are transcribed from non-protein-coding human genome and have more than 200 nt in length [4]. LncRNAs are increasingly functionally identified and experimentally consolidated to be related to tumor neoplasia and progression in diverse cancers [5]. LncRNA A1BG-AS1 inhibits cell proliferation and invasion in hepatocellular carcinoma via targeting miR-216a-5p [9]. Long intergenic non-protein coding RNA 992 (LINC00992) is a novel lncRNA that has been previously revealed to be elevated in tumors and substantiated as a master regulator for chemo-resistance [12]. No previous study has given a comprehensive explanation about the precise function or detailed mechanism of LINC00992 in prostate cancer
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