Abstract
The reactivities of the sulphydryl groups of minor component of duck hemoglobin with 5,51-dithiobis-(2-nitrobenzoic acid), DTNB, in the presence of inositol hexakisphosphate (inositol-P6) have been studied. The reaction has been monitored as a function of pH (pH 7.0 – 9.0) and at ionic strength of 50 mmolL-1. The carbonmonoxy and aquomet derivatives were used for the study. Biphasic time courses were obtained under all experimental conditions and the two kinetic phases were well separated along the time axis. From the observed pseudo-first order rate constants, kobs, the apparent second-order rate constants, kapp, were calculated. Inositol-P6 reduced the rates of all reactions for the two derivatives for both CysF9(93)β and CysB5(23)β. The reaction of CysF9(93)β gave an increase of 0.88 unit of pKa for the aquomet derivative but a decrease of 0.40 unit of pKa for the carbonmonoxy derivative. The salt-bridge formed between HisHC3 and AspFG1 is more strengthened by inositol-P6 in aquomet than in carbonmonoxy derivative. Key words: Duck, hemoglobin, sulphydryl, inositol-P6.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
