Leukotriene C4 contents, synthase and catabolic activity in human meningiomas.

Abstract

Leukotriene has been proposed as a factor of tumour induced brain oedema. Independently of its size, meningioma occasionally shows various extents of peritumoural oedema. We investigated LTC4 tissue contents, LTC4 catabolic and synthetic activity in 12 human meningiomas and their correlation with peritumoural oedema was studied. LTC4 contents were varied from 0.01 to 8.21 pg/mg tissue. When LTA4, an unstable expoxide intermediate was incubated with tumour homogenate, LTC4 was rapidly synthesized. However, LTC4 levels generated by incubating LTA4 with each homogenate were much different in each case. Degradation of LTC4 to LTD4, LTE4, and other polar materials was also rapid by incubation with tumour homogenates. Approximately 70% of added LTC4 was transformed to LTD4, LTE4 nor 6-trans LTB4 diastereoisomers during 30 min incubation at 37 degrees C. The results suggested that there were significant LTC4 tissue contents and LTC4 synthetic and catabolic activity in meningiomas. Oedema index ranged from 1.0 (no peritumoural oedema) to 67.5. No significant correlation, however, was observed not only between the LTC4 tissue contents and LTC4 synthetic or catabolic activities but also between each of these three parameters and peritumoural oedema. Thus, these results do not support a significant correlation of sulfidopeptide LTs with oedema formation in meningioma patients. Since leukotrienes are extremely unstable compounds, LTC4 tissue contents should be carefully discussed along with a consideration of rapid LTC4 synthesis and catabolism. Further role of leukotrienes in meningioma tissue should be studied.