Abstract
To investigate the role of Golgi phosphoprotein 3 (GOLPH3) in tumour growth and metastasis of esophageal squamous cancer. A lentiviral shRNA-vector was utilized to stably knockdown GOLPH3 in Eca-109 esophageal squamous cancer cells. mRNA transcription and protein expression of GOLPH3 were examined by real-time quantitative PCR and Western blotting, respectively. Cell proliferation activity was assessed by MTT assay and invasion and migration potentials by matrigel invasion and transwell motility assays. Stable knockdown in the GOLPH3 cell line was established. PD-A gene expression was significantly suppressed by lentivirus-mediated RNAi, which resulted in reducing the capacity for cell proliferation, migration, invasion and adhesion in vitro. In vivo, GOLPH3 depletion resulted in inhibition of tumour growth, with stable decrease in the expression of GOLPH3 in tumor xenografts. Our findings suggest that lentivirus mediated silencing of the GOLPH3 gene has a significant anti-tumour effect on esophageal squamous cancer in vitro and in vivo. In addition, the results indicate that GOLPH3 might be an effective molecular target for gene therapy in esophageal squamous cancer.
Highlights
Esophageal cancer is often refractory to current therapeutic approaches and has poor outcomes
Our findings suggest that lentivirus mediated silencing of the Golgi phosphoprotein 3 (GOLPH3) gene has a significant anti-tumour effect on esophageal squamous cancer in vitro and in vivo
Eca-109 cells were transfected with the lentivirus (LentiGOLPH3-sh or Lenti-GOLPH3-nc) for 48h, transfection efficiency observed by the fluorescent microscope were 60% (Figure 1A and B)
Summary
Esophageal cancer is often refractory to current therapeutic approaches and has poor outcomes. The cell biological, biochemical and functional analyses have shown that GOLPH3 can increase cell transformation and tumor growth by enhancing the activity of mTORC (Dippold et al, 2009; Wood et al, 2009; Scott and Chin 2010; Graham and Burd, 2011). The preliminary researches shown that GOLPH3 is relative with poor prognosis of some tumors, including gastric cancer (Hu et al, 2013) and esophageal squamous cell carcinoma (Wang et al, 2012). It is unclear whether inhibition of GOLPH3 can reduce tumor growth of esophageal squamous cell carcinoma
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