Abstract
In cell culture, the pH of the culture medium is one of the most important conditions. However, the culture medium may have non-uniform pH distribution due to activities of cells and changes in the environment. Although it is possible to measure the pH distribution with an existing pH meter using distributed electrodes, the method involves direct contact with the medium and would greatly increase the risk of contamination. Here in this paper, we propose a computed tomography (CT) scan for measuring pH distribution using the color change of phenol red with a light-emitting diode (LED) light source. Using the principle of CT scan, we can measure pH distribution without contacting culture medium, and thus, decrease the risk of contamination. We have developed the device with a LED, an array of photo receivers and a rotation mechanism. The system is firstly calibrated with different shapes of wooden objects that do not pass light, we succeeded in obtaining their 3D topographies. The system was also used for measuring a culture medium with two different pH values, it was possible to obtain a pH distribution that clearly shows the boundary.
Highlights
Our previous study shows that a cellular tissue cultured under periodic pressure would gain a greater elasticity than the ones without such pressurized environment [1,2]
We propose a novel method to measure the distribution of the pH in the cell culture medium by using the principle of computed tomography (CT) scan
We proposed a method that can measure the pH distribution in culture medium in non-contact
Summary
Our previous study shows that a cellular tissue cultured under periodic pressure would gain a greater elasticity than the ones without such pressurized environment [1,2]. To observe the time-lapse transition of pH of the cell culture medium, methods that obtain the pH quantitatively by measuring the color change of Phenol red added in the cell culture medium have been proposed [7,8,9,10,11,12]. In these methods, the sensor probe does not contact with the cell culture medium, the measurement would be contamination free. It cannot measure the distribution of the pH because it measures pH assuming that the pH of the culture medium is uniform in the whole area
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