Leaf carbon isotope discrimination, ash content and K relationships with seedcotton yield and lint quality in lines of Gossypium hirsutum L.

Abstract

In a two-year (2005–2006) study conducted at three sites in central (Larissa) and northern (Alexandroupolis, Thessaloniki) Greece, we aimed to investigate the relationships between seedcotton yield and lint quality with leaf physiological traits (carbon isotope discrimination- Δ, ash content and K concentration). Eighteen lines with their original cultivars ( Christina, Flora, Corona) were tested under the ultra-low density of 1.2 plants m −2. In combined data over years, a significant, positive correlation between seedcotton yield and Δ or ash content was found only in the driest and lowest-yielding site (Larissa), indicating that genotypes that keep their stomata open and in turn exhibit the highest Δ values, had an advantage in such environments. In pooled data from the two most productive sites (Thessaloniki and Alexandroupolis), which had the highest Δ and ash content values, evidenced a negative correlation between seedcotton yield and both physiological traits. Seedcotton yield was negatively related with leaf K concentration in Larissa and Alexandroupolis but no significant relationship was found in Thessaloniki where leaf K concentration was below adequacy limit. Δ was positively related with ash content which suggests that the latter could be a putative surrogate of Δ. Negative correlation between Δ and leaf K concentration was found in two out of three sites (Larissa and Thessaloniki) as well as between ash content and K in one site (Larissa). These findings suggest that K accumulation in leaves is not just a passive procedure via transpiration stream. Significant, linear relationships of each physiological trait between sites showed that genotypic ranking was constant in the three sites, an indication of heritability. Ash content had the highest significance levels and correlation coefficients. Even though significant genotypic differentiation was observed for the three physiological and two of the lint quality traits ( i.e. fibre length, micronaire) determined in Alexandroupolis, only a weak, negative relationship between fibre length and leaf K concentration was evident. In sum, leaf physiological traits ( Δ, ash content and K concentration) could not be reliably used for yield selection in cotton owing to site-specific effects, which prejudice the yield–physiological traits relationship. Ash content– Δ relationship merits further research in order the former to be established as a putative surrogate of Δ.