Abstract
The main aim of the work was to scientifically prove the anti-inflammatory property of the polyphenol-rich fraction of hydro-alcoholic leaf extract of Litsea quinqueflora (Dennst.) Suresh by protein denaturation and free radical scavenging activity. The polyphenol-rich fraction of hydro-alcoholic leaf extract was obtained via acid-alkali hydrolysis, followed by fractionation with chloroform and ethyl acetate. HPTLC profiling of the finally obtained ethyl acetate fraction and consequent derivatisation with aluminium chloride revealed the presence of flavonoids in a more purified form. LCMS/MS analysis tentatively identified the presence of bioactive polyphenolic compounds such as gallocatechin, sinapic acid, pinocembrin, paeonol and umbelliferone in the separated fraction. The polyphenol-rich fraction of hydro-alcoholic extract of leaves showed anti-denaturing activity in heat-induced bovine serum albumin denaturation with an IC50 value of 23.59 µg/ml and was statistically significant at 0.1% level. The antioxidant property of the polyphenol-rich fraction determined by its free radical scavenging ability against DPPH and ABTS showed IC50 values 122.98 and 135.44 µg/ml respectively and was also statistically significant at 0.1% level. Hence, the traditional use of Litsea quinqueflora as an anti-inflammatory agent can be attributed to the presence of polyphenols.
Highlights
Plant-derived medicines attracted the attention of researchers due to the presence of different phytochemicals having a multitude of health benefits to humans [1]
LCMS/MS analysis tentatively identified the presence of bioactive polyphenolic compounds such as gallocatechin, sinapic acid, pinocembrin, paeonol and umbelliferone in the separated fraction
The antioxidant property of the polyphenol-rich fraction determined by its free radical scavenging ability against DPPH and ABTS showed IC50 values 122.98 and 135.44 μg/ml respectively and was statistically significant at 0.1% level
Summary
Plant-derived medicines attracted the attention of researchers due to the presence of different phytochemicals having a multitude of health benefits to humans [1]. Among these phytochemicals, polyphenols are of much importance owing to their specific biological properties. These inevitable plant products play an important role in human health as they regulate metabolism, weight, cell proliferation and chronic diseases [3]. Biological properties such as anti-inflammatory, immunomodulatory, antioxidant, cardioprotective and anticancer activities can be attributed to polyphenols [4]. Traditional healers could identify the efficacy of plant-derived compounds, and they used them widely even before the advent of allopathy drugs [5]
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