Abstract
To improve the biological monitoring method for 2,6- and 2,4-toluenediisocyanate (TDI) exposure, we developed a simple and rapid method for analysis of the corresponding urinary metabolites, 2,6- and 2,4-toluenediamine (TDA) using liquid chromatograph-mass spectrometry (LC–MS). One ml of urine was hydrolyzed at 100 °C for 1.5 h with H 2SO 4. Alkalinized hydrolysate was extracted with dichloromethane (DCM) and analyzed by atmospheric pressure chemical ionization (APCI) LC–MS, in positive-ion mode. The mass spectra of TDA isomers showed the protonated molecule [M+H] +, at m/ z 123 as the base peak. Calibration curves of 2,6-TDA were linear up to 400 μg/l. TDA isomers in urine of exposed workers as determined by LC–MS correlated well with those obtained by gas chromatography-mass spectrometry. 2,6- and 2,4-TDA were not detected in non-exposed subjects, whereas exposed workers showed urinary levels up to 250 and 63 μg/l, respectively.
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