Abstract
AbstractEleutherococcus senticosus (Rupr. & Maxim.) Maxim. fruits (ESF) is a natural health food that has been widely used in traditional medicine. This study aimed to establish and validate a rapid and sensitive method for determining of six phenolic acids in plasma of normal and T2DM rats by ultra-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). Separation and quantification were performed on a Waters HSS T3 column (100 × 2.1 mm, 1.8 μm) with water (A) and methanol (B). The electrospray ionization (ESI) source was operated in negative ionization mode. Multiple reaction monitoring (MRM) was used to quantitatively analyzed hyperoside m/z 463.2→301.1, caffeic acid m/z 179.1→135.1, chlorogenic acid m/z 353.2→190.9, gallic acid m/z 169.0→125.1, protocatechuic acid m/z 153.1→109.1, and isofraxidin m/z 221.1→191.0, respectively. The hypoglycemic effect of ESF was verified by determining blood glucose and lipid levels in a type 2 diabetes model. This study laid the foundation for exploring ESF clinical guidelines in the future.
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