Abstract
This study focuses on saponin fraction from sea buckthorn (Elaeagnus rhamnoides (L.) A. Nelson) leaves. It has known that for example teas from sea buckthorn leaves have anti-obesity properties. The objective of our present experiments was to investigate both the chemical composition of saponin fraction, as well as their biological properties in different in vitro models (using human plasma, blood platelets, and peripheral blood mononuclear cells (PBMCs)). We observed that saponin fraction reduces plasma lipid peroxidation and protein carbonylation induced by H2O2/Fe. This fraction also decreased DNA oxidative damage induced by H2O2 in PBMCs. Regarding the cytotoxicity of saponin fraction (0.5–50 µg/mL) none was found to cause lysis of blood platelets, and PBMCs. Our results, for the first time indicate that saponin fraction from sea buckthorn leaves may be a new promising source of compounds for prophylaxis and treatment of diseases associated with oxidative stress.
Highlights
The results published by Skalski et al [5,6] points that butanolic extracts, phenolic fraction and non-polar fraction from leaves of sea buckthorn has antioxidant effect and affects the hemostasis system in vitro [5,6]
Results of LC-MS/MS analysis of the saponin fraction of sea buckthorn leaves are shown in Table 1; several small peaks were not listed in the table, when no fragmentation data was obtained
It is possible to observe chains of neutral losses of monosaccharides, as well as ions of potential triterpenoid aglycones in CID spectra of 18 constituents of the saponin fraction of sea buckthorn leaves, which confirms the tentative identification of these compounds
Summary
For many years the problem of various diseases, including cardiovascular diseases and cancers, which are associated with oxidative stress has been investigated [4]. This problem was attacked from various angles. The results published by Skalski et al [5,6] points that butanolic extracts, phenolic fraction (in which ellagitannins and flavonoids were the dominant compounds) and non-polar fraction (in which non-polar compounds were the dominant) from leaves of sea buckthorn has antioxidant effect and affects the hemostasis system in vitro [5,6]. Cardiovascular diseases are often associated with obesity, which may be reduced by natural anti-obesity agents
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