Abstract
Liquid chromatography electrospray ionization tandem mass spectrometric (LC-ESI-MS/MS) qualitative and quantitative analysis of different extracts from the aerial parts and roots of Alchemilla acutiloba led to the identification of phenolic acids and flavonoids. To the best of our knowledge, isorhamnetin 3-glucoside, kaempferol 3-rutinoside, narcissoside, naringenin 7-glucoside, 3-O-methylquercetin, naringenin, eriodictyol, rhamnetin, and isorhamnetin were described for the first time in Alchemilla genus. In addition, the antioxidant, anti-inflammatory and cytotoxic activity of all extracts were evaluated. The results clearly showed that among analyzed extracts, the butanol extract of the aerial parts exhibited the highest biological activity comparable with the positive controls used.
Highlights
The genus Alchemilla includes a large number of types in the Polish flora that are not easy to identify
Total phenolic content (TPC) for A. acutiloba extracts and subfractions was estimated using Folin–Ciocalteu reagent, and the results were expressed as gallic acid equivalents (GAE) per g of dry extract (DE) (Table 1)
The results showed that the aerial parts (AH)
Summary
The genus Alchemilla includes a large number of types in the Polish flora that are not easy to identify. Alchemilla herb has long been used in European and Asian folk medicine in diseases caused by poor metabolism as well as for the treatment of eczema, wounds, ulcers„ and gynaecological problems [1,2,3]. Due to the relatively high tannin content, the Alchemilla herb has an effect characteristic of this group of compounds. After oral administration of the extract, diarrhea is reduced or inhibited [4]. The astringent properties of tannins impede the penetration of fluid into the intestinal lumen, as well as stop bleeding from damaged capillaries, reduce inflammation of the mucous membranes of the gastrointestinal tract, and inhibit the growth of pathogenic bacteria strains [4]. Alchemilla herb extract acts on the skin and connective tissue, especially in damaged areas, such as scars after wounds and burns. Partial regeneration of the capillaries is observed, the spots and traits on the skin slowly disappear, and the normal immunity and elasticity of the epidermis are restored in these places [3,4]
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