Abstract
Laser scanning microscopy is emerging as a powerful imaging tool in cryobiology. The basic microscopy system can be combined with various imaging modalities including Raman spectroscopy, fluorescence correlation spectroscopy, fluorescence lifetime imaging, or multiphoton imaging. Multiphoton imaging can be used to study intracellular ice formation at the subcellular level. A Raman imaging modality can be used for chemical mapping of frozen samples. A Raman spectrum gives information about characteristic molecular vibrations of specific groups in molecules. Raman images can be used to determine the localization of intra- and extracellular constituents and the various forms of water in freeze-concentrated solutions. Spectra can be collected during freezing and thawing of a sample using a temperature-controlled sample holder. In this chapter, various advanced cryoimaging methods are described. Special emphasis is given on the different imaging modalities that can be used to study the various aspects of cryopreservation.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
