Abstract
Using a pulsed, tunable dye laser details of the fluorescence behavior of single, intact human red blood cells have been studied. The high sensitivity of the laser excited fluorescence allows the detection of potential-dependent uptake and release of dye added to the suspension of living erythrocytes. The membrane potential of the erythrocytes was changed by means of different concentrations of valinomycin and potassium chloride in the suspension medium. Dye concentrations as low as 10 -8 M in water have been detected in a layer of 10 μm thickness and a cross sectional area of 1 μm 2.
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