Abstract
Objectives: Fluorescence spectroscopy which can be used for optical tissue diagnosis of tumor pathology deserves special interest. The purpose of the work was to study blood plasma and tumor tissue of men with different forms of prostate tumors by using laser induced fluorescence. Blood plasma and tumor tissue of the patients with benign hyperplasia of the prostate (BHP), BHP with inflammation, BHP with high grade PIN (BHP with HGPIN) and adenocarcinoma of prostate (CaP) have been studied. Results: In case of blood plasma fluorescence, intensity of the plasma proteins corresponding peak (340 - 360 nm) was increasing in the following manner: control group → BHP → BHP with HGPIN → CaP. The intensity of the nicotinamide coenzymes correspond peak (440 - 460 nm) was increased in case of BHP with HGPIN and CaP patients, but decreased in case of BHP, compared to control. In case of tumor tissue, the changes of the collagen peak (390 - 400 nm) intensity have been revealed in all cases of prostate tumor tissues. These alterations point to altered collagen biosynthesis levels in different tumor tissues, that reflects the structural changes and characteristics of malignant transformation. Also the changes of the nicotinamide coenzymes peak (440 - 460 nm) intensity in all spectra of tumor tissues were observed. The highest intensity of the peak was observed in the spectra of BHP with HGPIN and in prostate cancer tissue. Conclusions: Alterations of the coenzymes peak intensities perfectly reflect and are in accordance with the specific energy metabolism of prostate epithelial cells. Normalization of fluorescent spectra from different forms of prostate tumor tissues has shown that, each form has typical spectral shape and ratio of fluorescence peaks intensities.
Highlights
Prostate cancer is the most common cancer in men [1] [2]
For blood plasma fluorescence studies we have studied 4 groups: Patients with benign hyperplasia of the prostate (BHP), prostate benign hyperplasia with High Grade Prostate Intraepithelial Neoplasia (HGPIN), adenocarcinoma of prostate (CaP) and control group consisted with practically healthy men
For tumor tissue fluorescence studies we have studied 4 groups: with benign hyperplasia of the prostate (BHP), benign hyperplasia of the prostate with inflammation, prostate benign hyperplasia with High Grade Prostate Intraepithelial Neoplasia (HGPIN) and adenocarcinoma of prostate (CaP)
Summary
Prostate cancer is the most common cancer in men [1] [2]. Prevention and successful treatment of the tumor pathology require the study of all risk factors and molecular mechanisms that influence progression of the disease.Investigation of energy metabolism alterations accompanying malignant transformation of prostate tissue, is of current importance, since in epithelial cells of prostate peripheral zone (peripheral zone comprises 70% of the prostate gland, and 80% of malignant transformation occurs just on this segment) energy transformation occurs diversely from other types of cancers [3].Most tumor cells demonstrate inefficient aerobic glycolysis with impaired Krebs cycle activity. Carcinogenesis in general is associated with metabolic transformation from energy efficient benign cells to energy inefficient cancer cells. From other Tumors, the complete activation of the Krebs cycle takes place (normally these cells reveal partial activity of the Krebs cycle because of high concentration of zinc) on the early stage of malignant transformation of the prostate glandular epithelium [1]. The metabolic switch from energy inefficient prostate benign epithelial cells to energy efficient malignant ones occur [4]. Such changes of energy metabolism in epithelial cells of prostate cancer is the subject of high interest, the mechanism of these changes has not been fully studied yet
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
