Abstract
Laser assisted microvascular anastomosis (LAMA) was performed on rat femoral arteries anddd veins using a milliwatt CO2 laser. The histological appearance of the anastomoses was assessed immediately after the procedure and at regular intervals up to seven months. There was extensive thermal-induced transmural necrosis. The weld at the anastomosis was shown to comprise of fibrin, which later organized into fibrous tissue. Re-endothelialization occurred earlier in veins, and the endothelial nature of the surface cells was confirmed by factor VIII-related antigen positivity by the avidin-biotin peroxidase complex technique. Healing was complete by 9 weeks, but the vessel was not reconstituted to its normal appearance. At the site of the anastomosis 20% of the vessels had false aneurysms.
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